CAJ | 학술논문

以北京市永定河王平湿地为研究对象,应用末端限制性片段长度多态性(T-RFLP)对王平湿地再生水补水口及其远离补水口的香蒲根际细菌和氨氧化细菌(AOB)的群落结构及多样性变化进行分析,在此基础上结合MiCA3比对、典范对应分析(CCA)和多元统计中因子分析(FA),解析湿地不同空间细菌群落功能特性的变化.结果显示再生水补水口细菌群落各多样性指数均明显低于远离补水口细菌群落,而氨氧化细菌群落则呈现相反趋势.基于传统的T-RFLP片段MiCA3比对、CCA和FA相结合的分析表明：对细菌群落,占再生水补水口细菌群落总丰度55.6%的菌群与150bp代表的鞘氨醇单胞菌(Sphingomonas sp.)具相似净化功能,并与总有机碳生物化学循环过程具密切关系,其次占再生水补水口上游细菌群落总丰度75.5%的菌群与81bp为代表的假单胞菌属(Pseudomonas sp.)和Geitlerinema sp.具相似净化功能,并与氮的生物化学循环过程具密切关系.占再生水补水口下游细菌群落总丰度68.7%的菌群与138bp代表的小单孢菌属(Micromonospora sp.)具相似净化功能,同时与重金属Cu、V、和Ti生物化学循环具密切关系.对氨氧化细菌,266bp受铵态氮影响较大,占再生水补水口氨氧化细菌群落总丰度65.5%的菌群与266bp代表的Nitrosomonas sp.具相似的生境特征,适合在高氨环境中生长；与58bp所代表的Nitrosospira sp.具相似功能特性菌群丰度在远离补水口样点中较前者呈现显著增加趋势,表征该类菌群适合在相对低氨环境中生长.
이북경시영정하왕평습지위연구대상,응용말단한제성편단장도다태성(T-RFLP)대왕평습지재생수보수구급기원리보수구적향포근제세균화안양화세균(AOB)적군락결구급다양성변화진행분석,재차기출상결합MiCA3비대、전범대응분석(CCA)화다원통계중인자분석(FA),해석습지불동공간세균군락공능특성적변화.결과현시재생수보수구세균군락각다양성지수균명현저우원리보수구세균군락,이안양화세균군락칙정현상반추세.기우전통적T-RFLP편단MiCA3비대、CCA화FA상결합적분석표명：대세균군락,점재생수보수구세균군락총봉도55.6%적균군여150bp대표적초안순단포균(Sphingomonas sp.)구상사정화공능,병여총유궤탄생물화학순배과정구밀절관계,기차점재생수보수구상유세균군락총봉도75.5%적균군여81bp위대표적가단포균속(Pseudomonas sp.)화Geitlerinema sp.구상사정화공능,병여담적생물화학순배과정구밀절관계.점재생수보수구하유세균군락총봉도68.7%적균군여138bp대표적소단포균속(Micromonospora sp.)구상사정화공능,동시여중금속Cu、V、화Ti생물화학순배구밀절관계.대안양화세균,266bp수안태담영향교대,점재생수보수구안양화세균군락총봉도65.5%적균군여266bp대표적Nitrosomonas sp.구상사적생경특정,괄합재고안배경중생장；여58bp소대표적Nitrosospira sp.구상사공능특성균군봉도재원리보수구양점중교전자정현현저증가추세,표정해류균군괄합재상대저안배경중생장.
Water reclamation and reuse have been actively promoted in Beijing, but the potential influences of reclaimed water on the structure and function of microbial community were still poorly understood. Therefore, communities of both bacterial and ammonia oxidizing bacterias in cattail rhizosphere between the samples of reclaimed water outfall (RWO) in the Wang ping Wetland of Yong ding River, Beijing and far from the reclaimed water outfall (FRWO) were compared. Terminal Restriction Fragment Length Polymorphism (T-RFLP) was conducted to quantitatively detect the changes of bacterial and ammonia bacterial community structures and diversity. Then, Microbial Community Analysis Ⅲ, Canonical Correspondence Analysis (CCA) and Factor Analysis (FA) were used to investigate the changes of bacterial and ammonia bacterial community functions in different water qualities. The results were as followed: samples were classified according to the disturbance degree of reclaimed water. The bacterial diversity of RWO was significantly lower than that of FRWO, while the ammonia oxidizing bacterial diversity of RWO was higher than that of FRWO. As for bacteria, the function of root-attached phylotypes in RWO samples, which accounted for 55.6% of the bacterial community, was similar to the Sphingomonas sp. by 150-bp T-RFs. This function might be closely related to the total organic carbon in the biological chemical cycle. The major phylotypes from upstream samples had the same function with Pseudomonas sp.and Geitlerinema sp. by 80-bp T-RFs, whose proportion was 75.5%of the bacterial community, and the function was closely related to nitrogen in the biological chemical cycle. The major phylotypes from downstream samples had the same function with Micromonospora sp.by138-bp T-RFs, whose proportion was 68.7% of the bacterial community, and the function was closely related to metals such as Cu, V and Ti in the biochemical cycle. For ammonia oxidizing bacteria, the function of root-attached phylotypes in RWO sample, which accounted for 65.5% of all the ammonium bacterial community, was similar to the Nitrosomonas sp. by 266-bpT-RFsliving in high ammonium environment. The major phylotypes from FRWO had the same function with Nitrosospira sp. by 58-bp T-RFs living in low ammonium environment.