CAJ | 학술논문

目的：探索β-TCP修复骨质疏松性大鼠骨缺损的效果。方法取SD大鼠20只，先去卵巢建立骨质疏松模型，等饲养3个月保证骨质疏松模型成功后再建立双侧大鼠股骨干骺端缺损模型，将手术成功的骨质疏松性骨缺损模型随机均分为两组，β-TCP植入组于骨缺损处植入β-TCP，空白对照组不作任何处理。术后4，8w进行大体观察、X射线摄片、组织病理学观察，Micro-CT观察。结果术后4、8w，β-TCP 植入组：缺损区X射线影像有明显的骨组织生成，HE染色可见大量骨小梁和较多核深染的长梭形骨细胞，Micro-CT显示骨微观结构修复效果较好，且能明显增加股骨骨密度；空白对照组X射线骨组织生成较少，骨小梁或骨板结构较少，排列较紊乱，Micro-CT显示骨微观结构修复效果不够理想，股骨骨密度增加不明显。结论β-TCP可较好地修复骨质疏松性大鼠股骨干骺端缺损。
목적：탐색β-TCP수복골질소송성대서골결손적효과。방법취SD대서20지，선거란소건립골질소송모형，등사양3개월보증골질소송모형성공후재건립쌍측대서고골간후단결손모형，장수술성공적골질소송성골결손모형수궤균분위량조，β-TCP식입조우골결손처식입β-TCP，공백대조조불작임하처리。술후4，8w진행대체관찰、X사선섭편、조직병이학관찰，Micro-CT관찰。결과술후4、8w，β-TCP 식입조：결손구X사선영상유명현적골조직생성，HE염색가견대량골소량화교다핵심염적장사형골세포，Micro-CT현시골미관결구수복효과교호，차능명현증가고골골밀도；공백대조조X사선골조직생성교소，골소량혹골판결구교소，배렬교문란，Micro-CT현시골미관결구수복효과불구이상，고골골밀도증가불명현。결론β-TCP가교호지수복골질소송성대서고골간후단결손。
Objective To investigate the role ofβ-TCP in bone defect repairing in osteoporotic rats.Methods Twenty SD rats were ovariectomized and fed for 3 months to establish the osteoporosis model.Subsequently, bone defects were created in bilateral femoral metaphysis by surgery.Successful osteoporosis bone defect models were divided into 2 groups, includingβ-TCP group and control group.β-TCP was embedded into bone defect in β-TCP group.No treatment was performed in control group.Gross observation, X-ray radiographs, tissue pathological observation, and Micro-CT observation were conducted 4 and 8 weeks after the surgery.Results X-ray radiographs showed that bone tissue growth was obvious in bone defect zone 4 weeks and 8 weeks after surgery inβ-TCP group.HE staining showed that there were considerable amount of trabeculae and spindle shaped, deep stained osteoblasts.Micro-CT showed that bone micro-structure was comparatively well repaired, and the bone mineral density of the femur increased significantly.X-ray radiographs showed few bone growth, few trabeculae or bone lamella structure, and irregular distribution in control group.Micro-CT indicated that bone micro-structure was not desirably repaired, and the increase of bone mineral density of the femoral was not obvious.Conclusion β-TCP can comparatively well repair femoral metaphysis bone defect in osteoporotic rats.