CAJ | 학술논문

目的：观察加味左归丸对体外培养的骨髓间充质干细胞（ Mesenchymal stem cells，MSCs）向成骨细胞分化的影响。方法采用全骨髓法（贴壁筛选法）培养大鼠MSCs，用加味左归丸含药血清诱导大鼠MSCs向成骨细胞分化，并进行形态学观察和免疫细胞化学技术检测。结果与空白对照组相比，加味左归丸组与经典诱导组均可以明显促进成骨表型化MSCs的矿化结节形成及I型胶原表达（ P＜0.05）。结论加味左归丸可明显促进大鼠MSCs向成骨细胞转化，优化骨组织工程的种子细胞体系，改善骨组织的质量，促进骨组织的再生。
목적：관찰가미좌귀환대체외배양적골수간충질간세포（ Mesenchymal stem cells，MSCs）향성골세포분화적영향。방법채용전골수법（첩벽사선법）배양대서MSCs，용가미좌귀환함약혈청유도대서MSCs향성골세포분화，병진행형태학관찰화면역세포화학기술검측。결과여공백대조조상비，가미좌귀환조여경전유도조균가이명현촉진성골표형화MSCs적광화결절형성급I형효원표체（ P＜0.05）。결론가미좌귀환가명현촉진대서MSCs향성골세포전화，우화골조직공정적충자세포체계，개선골조직적질량，촉진골조직적재생。
Objective To observe the effect of Zuogui pill on the osteoblast differentiation from mesenchymal stem cells ( MSCs) in vitro.Methods MSCs from SD rats were cultivated by using whole marrow method.MSCs were induced to differentiate into osteoblasts by addition of conditioned serum of Zuogui pill.Morphology was observed and Immunocytochemistry was performed. Results Compared with the blank control group, mineralized nodule formation and type I collagen expression were promoted in both Zuogui pill group and classical induction group (P<0.05).Conclusion Zuogui pill induces the differentiation of osteoblasts from MSCs.It can optimize the seed cell system, improve the quality of the bone tissue, and promote the regeneration of the bone tissue.