CAJ | 학술논문

目的：探讨补肾健脾化瘀方对去势大鼠股骨骨髓组织中雌激素相关受体α（ ERRα）和过氧化物酶体增殖物活化受体γ协同刺激因子-1α（ PGC-1α） mRNA表达的影响。方法40只7月龄SD雌性大鼠随机分成假手术组、模型组、中药组（补肾健脾化瘀方）、阿伦膦酸钠组。治疗12w后，小动物双能X线检测左侧股骨骨密度，HE染色观察胫骨近端骨形态，QPCR检测骨髓组织中ERRα、PGC-1αmRNA表达水平检测。结果模型组骨密度低于假手术组（ P＜0.05）；中药组与阿伦膦酸钠组骨密度高于模型组（P＜0.05），而假手术组、中药组与阿伦膦酸钠组之间无统计学差异。与假手术组相比，模型组骨髓组织中ERRαmRNA表达水平上升（P＜0.05），PGC-1αmRNA表达水平下降（P＜0.05）。予以药物干预后，与模型组相比，中药组骨髓组织中ERRαmRNA表达水平下降（P＜0.05），PGC-1αmRNA表达水平上升（P＜0.05）；而阿伦膦酸钠组骨髓组织中ERRαmRNA表达水平下降（P＜0.01），PGC-1αmRNA表达水平虽呈现增高的趋势，但无统计学差异（P＞0.05）。结论补肾健脾化瘀方可能是通过提高骨髓组织中PGC-1αmRNA表达水平，降低ERRαmRNA表达水平，从而提高去势大鼠骨密度。
목적：탐토보신건비화어방대거세대서고골골수조직중자격소상관수체α（ ERRα）화과양화물매체증식물활화수체γ협동자격인자-1α（ PGC-1α） mRNA표체적영향。방법40지7월령SD자성대서수궤분성가수술조、모형조、중약조（보신건비화어방）、아륜련산납조。치료12w후，소동물쌍능X선검측좌측고골골밀도，HE염색관찰경골근단골형태，QPCR검측골수조직중ERRα、PGC-1αmRNA표체수평검측。결과모형조골밀도저우가수술조（ P＜0.05）；중약조여아륜련산납조골밀도고우모형조（P＜0.05），이가수술조、중약조여아륜련산납조지간무통계학차이。여가수술조상비，모형조골수조직중ERRαmRNA표체수평상승（P＜0.05），PGC-1αmRNA표체수평하강（P＜0.05）。여이약물간예후，여모형조상비，중약조골수조직중ERRαmRNA표체수평하강（P＜0.05），PGC-1αmRNA표체수평상승（P＜0.05）；이아륜련산납조골수조직중ERRαmRNA표체수평하강（P＜0.01），PGC-1αmRNA표체수평수정현증고적추세，단무통계학차이（P＞0.05）。결론보신건비화어방가능시통과제고골수조직중PGC-1αmRNA표체수평，강저ERRαmRNA표체수평，종이제고거세대서골밀도。
Objective To explore the effect of kidney-tonifying, spleen-strengthening, and blood stasis removing recipe on the expression of ERRαand PGC-1αmRNA in the bone marrow of the rat femurs in an ovariectomized rat model.Methods Forty 7-month-old female SD rats were randomly divided into sham-operated group, ovariectomized ( OVX) group, herb group ( kidney-tonifying, spleen-strengthening, and blood stasis removing recipe) , and alendronate ( ALEN) group, with 10 rats per group.After a 12-week treatment, the bone mineral density ( BMD) of the left femur was measured using small animal special dual energy X-ray absorptiometry.Bone morphology of the proximal tibia was observed using HE staining.The expression of ERRαand PGC-1αmRNA of bone marrow was detected using quantitative PCR.Results Compared with that in sham-operated group, BMD in OVX group was obviously decreased ( P<0.05 ) .Compared with that in OVX group, BMD in herb group and in ALEN group was obviously increased ( P<0.05 ) .There was no statistically significant among sham-operated, herb and ALEN groups.Compared with sham-operated group, the expression of ERRαmRNA in OVX group was obviously increased (P<0.05) and the expression of PGC-1αmRNA was obviously decreased (P<0.05).After the drug intervention, the expression of ERRαmRNA in herb group was obviously decreased ( P<0.05 ) and the expression of PGC-1αmRNA was obviously increased compared with that in OVX group (P<0.05).The expression of ERRαmRNA in ALEN group was obviously decreased (P<0.01), but PGC-1αmRNA expression increased with no statistical difference (P>0.05).Conclusion The kidney-tonifying, spleen-strengthening, and blood stasis removing recipe enhances rat BMD by increasing the expression of PGC-1αmRNA and decreasing the expression of ERRαmRNA.