CAJ | 학술논문

目的：缺氧诱导因子-1α（hypoxia inducible factor-1α， HIF-1α）、p-糖蛋白（p-glycoprotein， p-gp）的表达，HPV感染在非小细胞肺癌（ non-small cell lung cancer ， NSCLC）发生发展中的作用及三者的相互关系尚不清楚。文中研究NSCLC中HIF-1α和p-gp的相关性，探讨HIF-1α和p-gp在NSCLC发生发展及多药耐药（ multidrug resistance ， MDR）中的作用，并初步分析HIF-1α和p-gp与人乳头状瘤病毒（ human papillomavirus ， HPV）感染的相关性。方法收集秦皇岛市第一医院2012年1-5月NSCLC新鲜标本60例作为NSCLC组，20例肺良性病变组织作为肺良性病变组，应用PCR方法选用HPV16、18型特异性引物检测2组中HPV DNA的表达，应用免疫组化法检测HIF-1α、p-gp的表达。结果 NSCLC 中HIF-1α阳性率为48．3％（29/60），肺良性病变未见阳性表达（χ2＝15．163，P＜0．05）；p-gp的阳性率为60．0％（36/60），肺良性病变未见阳性表达（χ2＝21．818，P＜0．05）；HIF-1α阳性者中p-gp的表达率明显高于HIF-1α阴性者，两者呈正相关（ r＝0．313，P＜0．05）；NSCLC组中HIF-1α表达与组织分化程度及淋巴结转移相关；p-gp表达与组织学分型相关，肺腺癌p-gp阳性表达率（83．3％）高于肺鳞癌（50．0％），差异有统计学意义（P ＜0．05）。 NSCLC 高分化型 HPV DNA 检出率（8．3％）低于中、低分化型（50．0％），差异有统计学意义（P＜0．05）。淋巴结转移者HPV DNA检出率（60．6％）高于无淋巴结转移者（23．3％），差异有统计学意义（P＜0．05）。结论 HIF-1α与p-gp参与了NSCLC的发生发展及MDR；HPV感染可能是NSCLC发生的病因学因素之一，但NSCLC中HIF-1α、p-gp表达与HPV感染均无协同作用。
목적：결양유도인자-1α（hypoxia inducible factor-1α， HIF-1α）、p-당단백（p-glycoprotein， p-gp）적표체，HPV감염재비소세포폐암（ non-small cell lung cancer ， NSCLC）발생발전중적작용급삼자적상호관계상불청초。문중연구NSCLC중HIF-1α화p-gp적상관성，탐토HIF-1α화p-gp재NSCLC발생발전급다약내약（ multidrug resistance ， MDR）중적작용，병초보분석HIF-1α화p-gp여인유두상류병독（ human papillomavirus ， HPV）감염적상관성。방법수집진황도시제일의원2012년1-5월NSCLC신선표본60례작위NSCLC조，20례폐량성병변조직작위폐량성병변조，응용PCR방법선용HPV16、18형특이성인물검측2조중HPV DNA적표체，응용면역조화법검측HIF-1α、p-gp적표체。결과 NSCLC 중HIF-1α양성솔위48．3％（29/60），폐량성병변미견양성표체（χ2＝15．163，P＜0．05）；p-gp적양성솔위60．0％（36/60），폐량성병변미견양성표체（χ2＝21．818，P＜0．05）；HIF-1α양성자중p-gp적표체솔명현고우HIF-1α음성자，량자정정상관（ r＝0．313，P＜0．05）；NSCLC조중HIF-1α표체여조직분화정도급림파결전이상관；p-gp표체여조직학분형상관，폐선암p-gp양성표체솔（83．3％）고우폐린암（50．0％），차이유통계학의의（P ＜0．05）。 NSCLC 고분화형 HPV DNA 검출솔（8．3％）저우중、저분화형（50．0％），차이유통계학의의（P＜0．05）。림파결전이자HPV DNA검출솔（60．6％）고우무림파결전이자（23．3％），차이유통계학의의（P＜0．05）。결론 HIF-1α여p-gp삼여료NSCLC적발생발전급MDR；HPV감염가능시NSCLC발생적병인학인소지일，단NSCLC중HIF-1α、p-gp표체여HPV감염균무협동작용。
Objective The effect of hypoxia inducible factor-1α( HIF-1α) , p-glycoprotein ( p-gp) and human papillomavir-us(HPV) infection on non-small cell lung cancer (NSCLC) and their interrelationship are still not clear .The article aimed to study the relationship between HIF-1αand P-gp, their role in the occurrence , development and multidrug resistance of NSCLC , as well as the in-terrelationship among HIF-1α, p-gp expression and HPV infection . Methods By using immunohistochemistry , the expression of HIF-1αand p-gp was detected in 60 NSCLC tissues and 20 benign lung disease tissues .PCR was employed to detect the expression of HPV16/18 DNA. Results The positive expression rate of HIF-1αand p-gp was 48.3% and 60.0% respectively in NSCLC, but there was no positive expression of HIF-1αand p-gp detected in the benign lung disease group (P<0.05).The expression of HIF-1αwas in positive corelation with p-gp in NSCLC (r=0.313, P<0.05).The detective rate of HPV DNA was 41.7% in NSCLC group and 5.0%in the benign lung disease group , and there was a significant difference between them (P<0.05).The positive expression rate of HIF-1αin HPV DNA positive group and negative group was 52.0% and 45.7% respectively, which showed no significant difference (P>0.05).The positive expression rate of p-gp in HPV DNA positive group and negative group was 64.0% and 57.1%respectively, which showed no significant difference too (P>0.05). Conclusion HIF-1αand p-gp are involved in the occurrence, development and multidrug resistance of NSCLC .HPV infection may be one of the etiological factors in the carcinogenesis of NSCLC . However , neither the HIF-1αexpression nor the p-gp expression is associated with HPV infection .