CAJ | 학술논문

目的：共刺激细胞是一种对肿瘤细胞有杀伤作用的 NK样T细胞，既往研究表明羽扇豆醇作为一种天然植物提取物，能改变NK细胞、γδT细胞的生长及其对肿瘤细胞的作用。文中主要探讨羽扇豆醇对人共刺激细胞杀伤结肠癌细胞株 SW480的影响。方法取健康人外周血单个核细胞在体外经多种细胞因子诱导为共刺激细胞；不同浓度的羽扇豆醇在作用于共刺激细胞及结肠癌细胞株不同时间段后，甲基偶唑蓝（ MTT）法检测羽扇豆醇对共刺激细胞及结肠癌细胞株 SW480生长的影响；乳酸脱氢酶（ LDH）法检测共刺激细胞对结肠癌细胞株SW480的杀伤活性。结果羽扇豆醇浓度在0．1～200．0μg/mL时对共刺激细胞的生长有促进作用，对结肠癌细胞株SW480有抑制作用；羽扇豆醇诱导后，共刺激细胞对SW480的杀伤活性增强，浓度为12．5 mg/L时与空白对照比较的差异有统计学意义（76％vs 40％， P＜0．05）。结论羽扇豆醇能促进共刺激细胞的增殖，抑制结肠癌细胞株SW480的生长，并能增加SW480对共刺激细胞的敏感性，增强共刺激细胞对SW480细胞株的杀伤能力。
목적：공자격세포시일충대종류세포유살상작용적 NK양T세포，기왕연구표명우선두순작위일충천연식물제취물，능개변NK세포、γδT세포적생장급기대종류세포적작용。문중주요탐토우선두순대인공자격세포살상결장암세포주 SW480적영향。방법취건강인외주혈단개핵세포재체외경다충세포인자유도위공자격세포；불동농도적우선두순재작용우공자격세포급결장암세포주불동시간단후，갑기우서람（ MTT）법검측우선두순대공자격세포급결장암세포주 SW480생장적영향；유산탈경매（ LDH）법검측공자격세포대결장암세포주SW480적살상활성。결과우선두순농도재0．1～200．0μg/mL시대공자격세포적생장유촉진작용，대결장암세포주SW480유억제작용；우선두순유도후，공자격세포대SW480적살상활성증강，농도위12．5 mg/L시여공백대조비교적차이유통계학의의（76％vs 40％， P＜0．05）。결론우선두순능촉진공자격세포적증식，억제결장암세포주SW480적생장，병능증가SW480대공자격세포적민감성，증강공자격세포대SW480세포주적살상능력。
Objective Co-stimulation cells is a kind of natual killer (NK)-like T cells, which can kill tumor cells.Previous studies show that lupeol , an natural plant extracts , can change the growth of NK cells ,γδT cells and their effects on tumor cells .This study aimed to investigate the effects of lupeol on human co-stimulation cells and colonic cancer cell lines SW 480 . Methods The peripheral blood mononuclear cells ( PBMC) from healthy volunteers were induced in vitro by different cytokines and transferred into Co-simulation cells.After SW480 and Co-stimulation cells were incubated with different concentrations of lupeol for different durations , methyl thiazolyl tetrazolium (MTT) was used to detect the effects of lupeol on co-stimulation cells and colonic cancer cell lines SW480. Lactate dehydrogenase was used to determine the cell-killing activity of Co-simulation cells to colonic cancer lines SW 480 . Results The concentration of lupeol in 0.1-200.0 mg/L promoted the growth of Co-stimulation cells and inhibited the colonic cancer cell lines SW480.When the concentration of lupeol is at 12.5 mg/L, the cell-killing activity of Co-simulation cells to colonic cancer lines SW 480 was enhanced significantly compared with the controls (76%vs 40%, P<0.05). Conclusion Lupeol could promote the prolifera-tion of Co-stimulation cells, inhibit the growth of cancer lines SW480, and strengthen the cytotoxicity of Co-stimulation cells against co-lonic cell lines SW480.