医学研究生学报
醫學研究生學報
의학연구생학보
JOURNAL OF MEDICAL POSTGRADUATE
2014年
10期
1042-1046
,共5页
苗药生仙汤%甲钴胺%马尾神经损伤%脑源性神经营养因子
苗藥生仙湯%甲鈷胺%馬尾神經損傷%腦源性神經營養因子
묘약생선탕%갑고알%마미신경손상%뇌원성신경영양인자
Miao medicine Sheng Xian Decoction%Mecobalamin%Cauda equina injury%Brain-derived neurotrophic factor
目的：脑源性神经营养因子（ brain derived neurophic factor ， BDNF）在马尾损伤修复中发挥了重要作用。文中拟观察苗药生仙汤对马尾神经损伤大鼠骶髓结构改变及BDNF表达的影响。方法采用神经夹持损伤的方式复制大鼠马尾神经损伤模型，按随机数字表法将大鼠分为正常对照组、空白对照组、苗药组和甲钴胺组，每组15只，各组于造模后第1天、第7天和第14天各处死5只大鼠。通过大鼠脊髓损伤功能（Basso-Beattie-Bresnahan， BBB）评分观察神经功能的恢复情况；HE染色观察大鼠骶髓结构改变；免疫组化染色观察大鼠骶髓内BDNF蛋白表达情况。结果 BBB评分：造模后第1天空白对照组评分、苗药组评分和甲钴胺组评分差异无统计学意义（P＞0．05）；造模后第7、14天，苗药组评分（17．0±1．00、19．60±0．55）和甲钴胺组评分（16．2±0．84、18．80±0．84）明显高于空白对照组（14．00±0．70、16．40±0．55），差异有统计学意义（P＜0．05），苗药组和甲钴胺组评分值差异无统计学意义（P＞0．05）；空白对照组、苗药组和甲钴胺组造模后评分值随时间均呈上升趋势，组内两两比较的差异有统计学意义（P＜0．05）。 HE染色：与空白对照组比较，苗药组和甲钴胺组大鼠骶髓破坏较轻，胶质细胞增生也明显改善，正常神经元数量较多。免疫组化染色：造模后第1天，空白对照组MOD值（0．152±0．007）、苗药组MOD值（0．156±0．008）和甲钴胺组MOD值（0．162±0．008）均明显高于正常对照组（0．130±0．006），差异有统计学意义（P＜0．05），其中，甲钴胺组MOD值明显高于苗药组和空白对照组，差异有统计学意义（P＜0．05），造模后第7、14天，苗药组MOD值（0．205±0．009、0．183±0．008）和甲钴胺组 MOD 值（0．217±0．033、0．187±0．007）明显高于空白对照组（0．181±0．007、0．161±0．014），差异有统计学意义（P＜0．05），苗药组和甲钴胺组之间MOD值差异无统计学意义（P＞0．05）。空白对照组、苗药组和甲钴胺组MOD值随时间推移均呈先升后降趋势；苗药组和甲钴胺组组内两两比较，差异有统计学意义（P＜0．05）。结论苗药生仙汤可提高机体BDNF表达，促进神经元存活，从而改善马尾神经损伤大鼠神经功能。
目的：腦源性神經營養因子（ brain derived neurophic factor ， BDNF）在馬尾損傷脩複中髮揮瞭重要作用。文中擬觀察苗藥生仙湯對馬尾神經損傷大鼠骶髓結構改變及BDNF錶達的影響。方法採用神經夾持損傷的方式複製大鼠馬尾神經損傷模型，按隨機數字錶法將大鼠分為正常對照組、空白對照組、苗藥組和甲鈷胺組，每組15隻，各組于造模後第1天、第7天和第14天各處死5隻大鼠。通過大鼠脊髓損傷功能（Basso-Beattie-Bresnahan， BBB）評分觀察神經功能的恢複情況；HE染色觀察大鼠骶髓結構改變；免疫組化染色觀察大鼠骶髓內BDNF蛋白錶達情況。結果 BBB評分：造模後第1天空白對照組評分、苗藥組評分和甲鈷胺組評分差異無統計學意義（P＞0．05）；造模後第7、14天，苗藥組評分（17．0±1．00、19．60±0．55）和甲鈷胺組評分（16．2±0．84、18．80±0．84）明顯高于空白對照組（14．00±0．70、16．40±0．55），差異有統計學意義（P＜0．05），苗藥組和甲鈷胺組評分值差異無統計學意義（P＞0．05）；空白對照組、苗藥組和甲鈷胺組造模後評分值隨時間均呈上升趨勢，組內兩兩比較的差異有統計學意義（P＜0．05）。 HE染色：與空白對照組比較，苗藥組和甲鈷胺組大鼠骶髓破壞較輕，膠質細胞增生也明顯改善，正常神經元數量較多。免疫組化染色：造模後第1天，空白對照組MOD值（0．152±0．007）、苗藥組MOD值（0．156±0．008）和甲鈷胺組MOD值（0．162±0．008）均明顯高于正常對照組（0．130±0．006），差異有統計學意義（P＜0．05），其中，甲鈷胺組MOD值明顯高于苗藥組和空白對照組，差異有統計學意義（P＜0．05），造模後第7、14天，苗藥組MOD值（0．205±0．009、0．183±0．008）和甲鈷胺組 MOD 值（0．217±0．033、0．187±0．007）明顯高于空白對照組（0．181±0．007、0．161±0．014），差異有統計學意義（P＜0．05），苗藥組和甲鈷胺組之間MOD值差異無統計學意義（P＞0．05）。空白對照組、苗藥組和甲鈷胺組MOD值隨時間推移均呈先升後降趨勢；苗藥組和甲鈷胺組組內兩兩比較，差異有統計學意義（P＜0．05）。結論苗藥生仙湯可提高機體BDNF錶達，促進神經元存活，從而改善馬尾神經損傷大鼠神經功能。
목적：뇌원성신경영양인자（ brain derived neurophic factor ， BDNF）재마미손상수복중발휘료중요작용。문중의관찰묘약생선탕대마미신경손상대서저수결구개변급BDNF표체적영향。방법채용신경협지손상적방식복제대서마미신경손상모형，안수궤수자표법장대서분위정상대조조、공백대조조、묘약조화갑고알조，매조15지，각조우조모후제1천、제7천화제14천각처사5지대서。통과대서척수손상공능（Basso-Beattie-Bresnahan， BBB）평분관찰신경공능적회복정황；HE염색관찰대서저수결구개변；면역조화염색관찰대서저수내BDNF단백표체정황。결과 BBB평분：조모후제1천공백대조조평분、묘약조평분화갑고알조평분차이무통계학의의（P＞0．05）；조모후제7、14천，묘약조평분（17．0±1．00、19．60±0．55）화갑고알조평분（16．2±0．84、18．80±0．84）명현고우공백대조조（14．00±0．70、16．40±0．55），차이유통계학의의（P＜0．05），묘약조화갑고알조평분치차이무통계학의의（P＞0．05）；공백대조조、묘약조화갑고알조조모후평분치수시간균정상승추세，조내량량비교적차이유통계학의의（P＜0．05）。 HE염색：여공백대조조비교，묘약조화갑고알조대서저수파배교경，효질세포증생야명현개선，정상신경원수량교다。면역조화염색：조모후제1천，공백대조조MOD치（0．152±0．007）、묘약조MOD치（0．156±0．008）화갑고알조MOD치（0．162±0．008）균명현고우정상대조조（0．130±0．006），차이유통계학의의（P＜0．05），기중，갑고알조MOD치명현고우묘약조화공백대조조，차이유통계학의의（P＜0．05），조모후제7、14천，묘약조MOD치（0．205±0．009、0．183±0．008）화갑고알조 MOD 치（0．217±0．033、0．187±0．007）명현고우공백대조조（0．181±0．007、0．161±0．014），차이유통계학의의（P＜0．05），묘약조화갑고알조지간MOD치차이무통계학의의（P＞0．05）。공백대조조、묘약조화갑고알조MOD치수시간추이균정선승후강추세；묘약조화갑고알조조내량량비교，차이유통계학의의（P＜0．05）。결론묘약생선탕가제고궤체BDNF표체，촉진신경원존활，종이개선마미신경손상대서신경공능。
Objective Brain -derived neurophic factor ( BDNF) has played an important role in the repair of cauda equina Injury.The aim of this article was to observe the influences of Miao medicine Sheng Xian Decoction on structural change and BDNF protein expression in the sacral spinal cord of rats with cauda equina injury . Methods The rat model of cauda equina injury was es-tablished by using nerve clamping .Rats were randomly divided into the following groups:normal group , control group , Miao medicine group and mecobalamin group , 15 rats in each group .According to the death time after modeling , the rats in each group were subdivi-ded into 1d group, 7d group and 14d group, 5 in each subgroup.The nerve function recovery of rats in every group was observed through Basso , Beattie & Bresnahan locomotor rating score ( BBB score ) .The structural change of sacral spinal cord was observed(11.40 ±1.14) and the mecobalamin group (11.40 ±0.89) (P>0.05).On the 7th, 14th day after modeling, the scores of the Miao medicine group (17.0 ±1.00, 19.60 ±0.55) and the mecobalamin group (16.2 ±0.84, 18.80 ±0.84) were much higher than the control group (14.00 ±0.70, 16.40 ±0.55) (P<0.05).There was no significant difference between the Miao medicine group and the mecobalamin group (P>0.05).The scores of the control group , the Miao medicine group and the mecobalamin group showed an increasing tendency with time, and the difference of scores among the groups are of statistical significance (P<0.05).HE staining:Compared with the normal group , the spinal cord structure of the control group was damaged and glial cell proliferation increased signif -icantly.Compared with the control group , rats in the Miao medicine group and the mecobalamin group were lightly damaged in spinal cord and got improvement in the glial cell proliferation as well as an increase in the number of normal neurons .Immunohistochemistry staining:On the 1st day after modeling, the MOD values of the control group (0.152 ±0.007) , the Miao medicine group (0.156 ± 0.008) and the mecobalamin group (0.162 ±0.008) were much higher than that of the normal group (0.130 ±0.006), and the difference was of significant difference (P<0.05).Moreover,the MOD value of the mecobalamin group was much higher than those of the control group and the Miao medicine group , which was of significant difference (P<0.05); there was no statistical significance between the control group and the Miao medicine group (P>0.05).On the 7th and 14th day after modeling, the MOD values of the Miao medicine group (0.205 ±0.009, 0.183 ±0.008) and the mecobalamin group (0.217 ±0.033, 0.187 ±0.007) were much higher than that of the control group (0.181 ±0.007, 0.161 ±0.014), which was of significant difference (P<0.05).There was no statistical significance between the values of Miao medicine group and the mecobalamin group (P>0.05).The MOD values of the control group , the Miao medicine group and the mecobalamin group showed a tendency of increase followed by decrease with time .The MOD values of 3 subgroups in the Miao medicine group and the mecobalamin group had no statistical significance (P>0.05).The MOD value of 14d subgroup was much lower than 7d subgroup in the control group (P<0.05), however, compared with 1d subgroup in the control group, there was no significant difference (P>0.05).There was no significant difference among the MOD value of 3 subgroups in the normal group (0.130 ±0.006, 0.130 ±0.006, 0.130 ±0.008) (P>0.05). Conclusion Miao medicine Sheng Xian Decoction can promote neuron survival and improve nerve functions through increasing the BDNF expression in the sacral spinal cord of rats with cauda equina injury .
