CAJ | 학술논문

几丁质酶广泛存在于高等植物体内，它可以分解真菌细胞壁中的几丁质，破坏其细胞结构，从而获得对真菌的抗性。试验以转苦瓜几丁质酶基因烟草（T-Chit）为供试植物，测定植株根系和叶片内几丁质酶活性，选取了3种具有代表性的真菌：毛霉、木霉、禾谷镰刀霉，通过抑菌试验验证了T-Chit组织萃取液对真菌的抗性。结果表明：1）T-Chit几丁质酶活性显著高于非转基因烟草（Nt-X），且转基因烟草根系几丁质酶活性比叶片高86％；2）T-Chit组织萃取液对毛霉生长具有明显抑制作用，根系强于叶片；3）T-Chit对木霉和禾谷镰刀霉的抑制具有时空效应：抑菌效果与植株部位及作用时间有关。
궤정질매엄범존재우고등식물체내，타가이분해진균세포벽중적궤정질，파배기세포결구，종이획득대진균적항성。시험이전고과궤정질매기인연초（T-Chit）위공시식물，측정식주근계화협편내궤정질매활성，선취료3충구유대표성적진균：모매、목매、화곡렴도매，통과억균시험험증료T-Chit조직췌취액대진균적항성。결과표명：1）T-Chit궤정질매활성현저고우비전기인연초（Nt-X），차전기인연초근계궤정질매활성비협편고86％；2）T-Chit조직췌취액대모매생장구유명현억제작용，근계강우협편；3）T-Chit대목매화화곡렴도매적억제구유시공효응：억균효과여식주부위급작용시간유관。
Chitinase widely exists in higher plants .It is able to degrade chitin in the cell walls of fungi , thus destroying the fungal cell structure .Depending on this functional mechanism ,plants gain resistance to fungi .In this study , transgenic tobacco has been transformed with chitinase gene (T-Chit ) of bal-sampear as experimental plants ,and we have measured the chitinase activity in roots and leaves ,and dem-onstrated the resistance of T-Chit tissue extract against Mucor racemosus sp ., Trichoderma viride sp ., and Fusarium graminearum sp .The results show that (1) Chitinase activity of T-Chit is greater than pa-rental tobacco plants (Nt-X) ,and in transgenic tobacco ,chitinase activity in root is 86% higher than that in leaf blade ;(2) the extract of T-Chit has strong antifungal effects against Mucor racemosus sp .,and re-sistance of root system is stronger than that of leaf blade ;(3) the resistance of T-Chit tissue extract has space-time effect on Trichoderma viride sp .and Fusarium graminearum sp .,and the effect varies with plant sites and action time .