绿色科技
綠色科技
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LVSE DASHIJIU
2014年
10期
83-85
,共3页
抗坏血酸过氧化物酶%非生物逆境%活性氧%基因表达
抗壞血痠過氧化物酶%非生物逆境%活性氧%基因錶達
항배혈산과양화물매%비생물역경%활성양%기인표체
ascorbate peroxidase%abiotic stress%reactive oxygen species%gene expression
指出了植物抗坏血酸过氧化物酶(Ascorbate peroxidase ,APX)含有高度保守的氨基酸残基,分为叶绿体型同工酶和非光合器官型同工酶,以各自特异方式调控细胞内过氧化氢(H2 O2)浓度。A PX基因编码区同源性较高,表达具有组织特异性,响应H2 O2、热激转录因子(Heat shock factors ,HSFs)等信号因子调节,可调控胁迫相关蛋白和转录因子的表达。非生物逆境胁迫下,过量表达A PX ,可有效清除物体内活性氧(reactive oxygen species ,ROS),减轻过氧化损伤,提高植物抵御逆境的能力。
指齣瞭植物抗壞血痠過氧化物酶(Ascorbate peroxidase ,APX)含有高度保守的氨基痠殘基,分為葉綠體型同工酶和非光閤器官型同工酶,以各自特異方式調控細胞內過氧化氫(H2 O2)濃度。A PX基因編碼區同源性較高,錶達具有組織特異性,響應H2 O2、熱激轉錄因子(Heat shock factors ,HSFs)等信號因子調節,可調控脅迫相關蛋白和轉錄因子的錶達。非生物逆境脅迫下,過量錶達A PX ,可有效清除物體內活性氧(reactive oxygen species ,ROS),減輕過氧化損傷,提高植物牴禦逆境的能力。
지출료식물항배혈산과양화물매(Ascorbate peroxidase ,APX)함유고도보수적안기산잔기,분위협록체형동공매화비광합기관형동공매,이각자특이방식조공세포내과양화경(H2 O2)농도。A PX기인편마구동원성교고,표체구유조직특이성,향응H2 O2、열격전록인자(Heat shock factors ,HSFs)등신호인자조절,가조공협박상관단백화전록인자적표체。비생물역경협박하,과량표체A PX ,가유효청제물체내활성양(reactive oxygen species ,ROS),감경과양화손상,제고식물저어역경적능력。
Plant ascorbate peroxidase contains highly conserved amino acid residues ,w hich divides into chloroplastid and non -photosynthetic isozymes that respectively regulate the concentration of H2O2 in cell .The APX gene's coding region has high homology and its expression shows tissue specificity ,APX gene responds to H2 O2 ,heat shock factors and other signal factors ,and it regulates and forces expressions of related proteions and transprction factors . Forced by abiotic stress ,over-expression of APX could effectively scavenge the ROS and alleviate the injury from peroxidation ,w hich improve plant's ability of stress resistance .