江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2014年
10期
104-108
,共5页
桔梗%高效液相色谱%指纹图谱
桔梗%高效液相色譜%指紋圖譜
길경%고효액상색보%지문도보
Platycodon grandiflorum%HPLC%Fingerprint
目的：建立商洛桔梗药材的高效液相色谱（ HPLC）指纹图谱，为商洛桔梗药材的道地性研究提供可靠资料。方法：采用HPLC法测定来源于商洛7个县（区）的39个桔梗样品。色谱条件：Hypersil C18柱（250 mm ×4．6 mm，5μm），以乙腈和H3 PO4混合溶液作为流动相，采用梯度洗脱方式，流速为0．5 mL/min，检测波长为210 nm，柱温30℃。结果：建立了39批桔梗药材的HPLC指纹图谱，找到18个共有特征峰。结论：该方法简单，重现性好，可以用于商洛桔梗药材的品质鉴定与评价。
目的：建立商洛桔梗藥材的高效液相色譜（ HPLC）指紋圖譜，為商洛桔梗藥材的道地性研究提供可靠資料。方法：採用HPLC法測定來源于商洛7箇縣（區）的39箇桔梗樣品。色譜條件：Hypersil C18柱（250 mm ×4．6 mm，5μm），以乙腈和H3 PO4混閤溶液作為流動相，採用梯度洗脫方式，流速為0．5 mL/min，檢測波長為210 nm，柱溫30℃。結果：建立瞭39批桔梗藥材的HPLC指紋圖譜，找到18箇共有特徵峰。結論：該方法簡單，重現性好，可以用于商洛桔梗藥材的品質鑒定與評價。
목적：건립상락길경약재적고효액상색보（ HPLC）지문도보，위상락길경약재적도지성연구제공가고자료。방법：채용HPLC법측정래원우상락7개현（구）적39개길경양품。색보조건：Hypersil C18주（250 mm ×4．6 mm，5μm），이을정화H3 PO4혼합용액작위류동상，채용제도세탈방식，류속위0．5 mL/min，검측파장위210 nm，주온30℃。결과：건립료39비길경약재적HPLC지문도보，조도18개공유특정봉。결론：해방법간단，중현성호，가이용우상락길경약재적품질감정여평개。
This study aimed to build the high performance liquid chromatography ( HPLC) fingerprint of authentic medicinal material Platycodon grandiflorum in Shangluo , and to provide reliable data for the geoherbalism study of this medicinal material . Thirty-nine Platycodon grandiflorum samples from 7 counties ( districts) of Shangluo city were determined by using HPLC under the following chromatographic conditions:using Hypersil C18 column (250 mm ×4.6 mm, 5 μm), mobile phase being the mixed solution of acetonitrile and H 3 PO4 , adopting gradient elution mode , flow velocity being 0.5 mL/min, detecting wavelength being 210 nm, and column temperature being 30 ℃.Finally, we built the HPLC fingerprint of 39 batches of Platycodon grandiflorum, and found out 18 common characteristic peaks .It was concluded that this method was simple with good repeatability , and could be used for the identification and evaluation of authentic medicinal material Platycodon grandiflorum in Shangluo .
