CAJ | 학술논문

以蓝莓“夏普蓝”品种为试验材料研究了组培苗繁殖技术。在培养基中添加不同浓度ZT、蔗糖，分析了其对组培苗丛生芽诱导增殖的影响，以及不同IBA浓度对组培苗生根的影响。结果表明：改良WPM＋ZT 1．25 mg/L培养条件下，“夏普蓝”组培苗丛生芽分化效果最佳。在培养基中附加蔗糖是蓝莓增殖培养所必需的，蔗糖浓度过高或过低，都可能对蓝莓增殖产生不良影响，以蔗糖40 g/L时的增殖效果最好；生长素IBA对“夏普蓝”的生根诱导有促进作用，IBA 0．6 mg/L对诱导蓝莓组培苗生根最有效，生根率可达88％，生根速度较快，粗细均匀，根系壮。
이람매“하보람”품충위시험재료연구료조배묘번식기술。재배양기중첨가불동농도ZT、자당，분석료기대조배묘총생아유도증식적영향，이급불동IBA농도대조배묘생근적영향。결과표명：개량WPM＋ZT 1．25 mg/L배양조건하，“하보람”조배묘총생아분화효과최가。재배양기중부가자당시람매증식배양소필수적，자당농도과고혹과저，도가능대람매증식산생불량영향，이자당40 g/L시적증식효과최호；생장소IBA대“하보람”적생근유도유촉진작용，IBA 0．6 mg/L대유도람매조배묘생근최유효，생근솔가체88％，생근속도교쾌，조세균균，근계장。
The propagation techniques of tissue -culture seedlings of blueberry variety “Xiapulan” were studied .The influ-ences of various media containing different concentrations of ZT and sucrose on the induced propagation of cluster buds of the tissue-culture seedlings , as well as the effects of different concentrations of IBA on the rooting of the tissue -culture seedlings were ana-lyzed.The results showed that the differentiation of cluster buds of “Xiapulan” tissue-culture seedlings was the best on the im-proved WPM+ZT 1.25 mg/L medium.It was necessary for the propagation of blueberry to add sucrose into the medium , but over-high or too low concentration of sucrose could cause adverse effect on the propagation of blueberry , and 40 g/L sucrose had the best effect.The auxin IBA had promotive action on the rooting of tissue -culture seedlings of “Xiapulan”, and 0.6 mg/L IBA had the best rooting effect:the rooting rate reached 88%, the rooting speed was relatively high , and the roots were strong and uniform in thickness .