CAJ | 학술논문

探讨新型氨基化小分子半抗原直接包被ELISA方法检测食品中氯霉素残留的可行性。采用氨基化氯霉素直接包被在经戊二醛活化后的酶标板表面，并通过生物素-链霉亲和素放大系统进一步提高该检测方法的灵敏度。结果表明，氨基化小分子半抗原直接包被ELISA的灵敏度和最低检出限分别为12.9 ng/mL和0.7 ng/mL，CV%为1.8%~4.5%。新型氨基化小分子半抗原直接偶联ELISA具有灵敏度高，干扰少，检测步骤简便、操作安全，测定结果较准确可靠。
탐토신형안기화소분자반항원직접포피ELISA방법검측식품중록매소잔류적가행성。채용안기화록매소직접포피재경무이철활화후적매표판표면，병통과생물소-련매친화소방대계통진일보제고해검측방법적령민도。결과표명，안기화소분자반항원직접포피ELISA적령민도화최저검출한분별위12.9 ng/mL화0.7 ng/mL，CV%위1.8%~4.5%。신형안기화소분자반항원직접우련ELISA구유령민도고，간우소，검측보취간편、조작안전，측정결과교준학가고。
The novel ELISA was developed for monitoring chloramphenicol remains in food by the directly attaching small molecule hapten with amino groups to the microtiter plate surface. chloramphenicol with amino-groups was directly coated on the surface of microtiter plates by treating with glutaraldehyde solution , and biotin-streptavidin system was employed to improve the sensitivity of immunoassay. The sensitivity of the new ELISA was 12.9 ng/mL, CV%was 1.8%-4.5%. The novel amination small molecule hapten coupled directly ELISA has many advantages, such as high sensitivity, less interference, simple operation and so on.