CAJ | 학술논문

研究银耳多糖的抗氧化活性。以银耳为原料，经简单分离纯化，制得银耳多糖。将ICR小鼠随机分为空白对照组、模型对照组、银耳多糖低、中、高剂量组，通过每日皮下注射D-半乳糖建立亚急性衰老小鼠模型，空白对照组用生理盐水代替。剂量组小鼠灌胃剂量分别为100、200、400 mg/（kg·bw），空白对照组和模型对照组用生理盐水代替，每日一次，持续八周。测定比较血清、肝脏、脑组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）与谷胱甘肽过氧化物酶（GSH-Px）活性、丙二醛（MDA）含量、总抗氧化能力（T-AOC）和皮肤中的羟脯氨酸含量的差异。与模型组相比，银耳多糖剂量组小鼠血清、肝脏、脑组织中的SOD、CAT、GSH-Px活性和总抗氧化能力显著增强，MDA含量的异常上升受到明显抑制，皮肤中的羟脯氨酸含量显著提高，且以银耳多糖中、高剂量组的效果更好。银耳多糖对D-半乳糖致亚急性衰老小鼠具有较强的抗氧化活性。
연구은이다당적항양화활성。이은이위원료，경간단분리순화，제득은이다당。장ICR소서수궤분위공백대조조、모형대조조、은이다당저、중、고제량조，통과매일피하주사D-반유당건립아급성쇠로소서모형，공백대조조용생리염수대체。제량조소서관위제량분별위100、200、400 mg/（kg·bw），공백대조조화모형대조조용생리염수대체，매일일차，지속팔주。측정비교혈청、간장、뇌조직중초양화물기화매（SOD）、과양화경매（CAT）여곡광감태과양화물매（GSH-Px）활성、병이철（MDA）함량、총항양화능력（T-AOC）화피부중적간포안산함량적차이。여모형조상비，은이다당제량조소서혈청、간장、뇌조직중적SOD、CAT、GSH-Px활성화총항양화능력현저증강，MDA함량적이상상승수도명현억제，피부중적간포안산함량현저제고，차이은이다당중、고제량조적효과경호。은이다당대D-반유당치아급성쇠로소서구유교강적항양화활성。
To explore the antioxidant activity of polysaccharides from tremella in vivo. The polysaccharides were obtained from tremella after being separated and purificated. ICR mice were randomly assigned into Normal Group, Control Group, Low, Middle and High dose Group of polysaccharides from tremella. Aging model mice were established by subcutaneous injection of D-galactose. Normal mice were treated in the same manner expect that sterile saline instead of D-galactose. Model mice were treated by different dosages of polysaccharides (100, 200, 400 mg/(kg·bw)) for successive 8 weeks, blank and control mice were given the different volumes of saline according to the weight of the mice. The contents of malonialdehyde (MDA), the activities of superoxide dismutase (SOD), catalase(CAT), glutathione peroxidase(GSH-Px) and total antioxidant capacity (T-AOC) in serum, brain and liver tissues were observed and hydroxyproline contents in skin were also determinated. Polysaccharides from tremella could significantly enhance the activities of SOD, CAT, GSH-Px and T-AOC in serum, brain and liver tissues, reduce MDA contents and raise hydroxyproline contents markedly in a dose-dependent manner. The higher dosages of polysaccharides performed better. polysaccharides from tremella possessed a strong antioxidant activity in D-galactose induced aging mice.