食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
19期
106-111
,共6页
韩瑨%吴正钧%季红%高彩霞%游春苹
韓瑨%吳正鈞%季紅%高綵霞%遊春蘋
한진%오정균%계홍%고채하%유춘평
肠膜明串珠菌Leuco 4%脱脂乳蔗糖培养基%产糖条件优化
腸膜明串珠菌Leuco 4%脫脂乳蔗糖培養基%產糖條件優化
장막명천주균Leuco 4%탈지유자당배양기%산당조건우화
Leuconostoc mesenteroides Leuco 4%skimmed milk-sucrose media%polysaccharide
以M17蔗糖培养基为筛选模型，从云南泡菜中筛选得到一株高产多糖的菌株Leuco 4，经鉴定为肠膜明串珠菌（CGMCC NO.6432）。对该菌株进行产糖条件优化实验后发现：在脱脂乳浓度10%（质量分数）、蔗糖浓度5%（w/v）、接种量2%（v/w）、发酵温度28℃的条件下，180 r/min摇床培养80 h后发酵液中多糖含量可达到11 g/L以上。
以M17蔗糖培養基為篩選模型，從雲南泡菜中篩選得到一株高產多糖的菌株Leuco 4，經鑒定為腸膜明串珠菌（CGMCC NO.6432）。對該菌株進行產糖條件優化實驗後髮現：在脫脂乳濃度10%（質量分數）、蔗糖濃度5%（w/v）、接種量2%（v/w）、髮酵溫度28℃的條件下，180 r/min搖床培養80 h後髮酵液中多糖含量可達到11 g/L以上。
이M17자당배양기위사선모형，종운남포채중사선득도일주고산다당적균주Leuco 4，경감정위장막명천주균（CGMCC NO.6432）。대해균주진행산당조건우화실험후발현：재탈지유농도10%（질량분수）、자당농도5%（w/v）、접충량2%（v/w）、발효온도28℃적조건하，180 r/min요상배양80 h후발효액중다당함량가체도11 g/L이상。
A bacterial strain Leuco-4 capable of expressing high yield of exopolysaccharide on M17-sucrose agar was isolated from pickles of Yunnan and was identified as Leuconostoc mesenteroides (CGMCC NO.6432). The optimized conditions for Leuco 4 to biosynthesize polysaccharides was found to be a combination of 10%(w/w) skimmed milk, sucrose concentration of 5%(w/v), inoculation ratio of 2%(v/w), fermentation temperature of 28 ℃, respectively. After cultivated aerobically at shaker of 180 r/min for 80 hours under the optimized conditions, the polysaccharide in the fermentation broth of L. mesenteroides Leuco-4 reached 11 g/L.
