中国民族民间医药
中國民族民間醫藥
중국민족민간의약
CHINESE JOURNAL OF ETHNOMEDICINE AND ETHNOPHARMACY
2014年
20期
10-11
,共2页
葛海生%姚红伟%王晓飞%于玲%柴江平
葛海生%姚紅偉%王曉飛%于玲%柴江平
갈해생%요홍위%왕효비%우령%시강평
高效液相色谱法%甘麦宁心胶囊%梓醇%含量测定
高效液相色譜法%甘麥寧心膠囊%梓醇%含量測定
고효액상색보법%감맥저심효낭%재순%함량측정
HPLC%GanMaining capsule%Catalpol%Content determination
目的:建立测定甘麦宁心胶囊中梓醇的含量测定方法。方法:采用 Aglilent 1200 SHISEIDO C18(4.6mm ×250mm,5μm),流动相为乙腈:0.1%磷酸水溶液(1:99),检测波长为210nm,流速为1.0ml /min,柱温为30℃。结果:梓醇在0.02~0.6μg/μl 范围内线性关系良好,平均回收率为99.43%(RSD =1.21%)。结论:本法简便,精密度高,重复性好,选择性佳,可用于甘麦宁心胶囊的质量控制。
目的:建立測定甘麥寧心膠囊中梓醇的含量測定方法。方法:採用 Aglilent 1200 SHISEIDO C18(4.6mm ×250mm,5μm),流動相為乙腈:0.1%燐痠水溶液(1:99),檢測波長為210nm,流速為1.0ml /min,柱溫為30℃。結果:梓醇在0.02~0.6μg/μl 範圍內線性關繫良好,平均迴收率為99.43%(RSD =1.21%)。結論:本法簡便,精密度高,重複性好,選擇性佳,可用于甘麥寧心膠囊的質量控製。
목적:건립측정감맥저심효낭중재순적함량측정방법。방법:채용 Aglilent 1200 SHISEIDO C18(4.6mm ×250mm,5μm),류동상위을정:0.1%린산수용액(1:99),검측파장위210nm,류속위1.0ml /min,주온위30℃。결과:재순재0.02~0.6μg/μl 범위내선성관계량호,평균회수솔위99.43%(RSD =1.21%)。결론:본법간편,정밀도고,중복성호,선택성가,가용우감맥저심효낭적질량공제。
Objective To establish the method for determination of Catalpol in GanMaining capsule.Method Catalpol were deter-mined by HPLC on C18 -Column at 210 nm,with acetonitrile -0.1% phosphoric acid solution (1∶99)as mobile phase,the flow rate were 1.0 ml/min.Result The linear range of Catalpol was 0.02 ~0.6μg/μl.The average recovery was 99.43%,RSD was 1.21%.Conclusion The method is simple,accurate and repeatable,which can be used for determination of Catalpol in GanMaining capsule.