CAJ | 학술논문

目的：探讨骨髓间充质干细胞（MSCs）移植在椎间盘退变修复过程中表皮生长因子（EGF）、血小板衍生因子（PDGF）与低氧诱导因子（HIF）-1α、Ⅱ型胶原的量效关系。方法用细针抽吸髓核构建兔椎间盘退变模型，体外培养MSCs，并采用绿色荧光蛋白（GFP）标记的腺病毒载体转染，标记MSCs。在模型构建成功2周后以每毫升5×106个细胞移植入退变椎间盘内。1、2、4、8周末用免疫荧光双染，并用激光共聚焦显微镜观测不同时间点MSCs中EGF、PDGF、HIF-1α、Ⅱ型胶原的表达及其变化规律。结果移植后MSCs中EGF、PDGF的表达在2周时达高峰，分别为（28.92±5.66）%、（40.77±8.13）%，随后下降；HIF-1α、Ⅱ型胶原缓慢上升，8周时达峰值，分别为（55.35±14.06）%、（37.23±9.53）%，2～4周时上升速度最快。结论早期（2周时）MSCs以分泌营养细胞因子效应为主，后期（4周后）以发挥自我分化效应为主。
목적：탐토골수간충질간세포（MSCs）이식재추간반퇴변수복과정중표피생장인자（EGF）、혈소판연생인자（PDGF）여저양유도인자（HIF）-1α、Ⅱ형효원적량효관계。방법용세침추흡수핵구건토추간반퇴변모형，체외배양MSCs，병채용록색형광단백（GFP）표기적선병독재체전염，표기MSCs。재모형구건성공2주후이매호승5×106개세포이식입퇴변추간반내。1、2、4、8주말용면역형광쌍염，병용격광공취초현미경관측불동시간점MSCs중EGF、PDGF、HIF-1α、Ⅱ형효원적표체급기변화규률。결과이식후MSCs중EGF、PDGF적표체재2주시체고봉，분별위（28.92±5.66）%、（40.77±8.13）%，수후하강；HIF-1α、Ⅱ형효원완만상승，8주시체봉치，분별위（55.35±14.06）%、（37.23±9.53）%，2～4주시상승속도최쾌。결론조기（2주시）MSCs이분비영양세포인자효응위주，후기（4주후）이발휘자아분화효응위주。
Objective To study the dose effect of epidermal growth factor(EGF),platelet-derived growth factor(PDGF) and hypoxia-inducible factor(HIF)-1α,typeⅡcollagen in transplantation of mesenchymal stem cells(MSCs) during the repair of disc degeneration. Methods A rabbit model of disc degeneration was developed by fine-needle nucleus aspiration,the MSCs was cultured in vivo and transfected by adenovirus vector labeled with green fluorescent protein(GFP). 2 weeks after successful devel-opment of rabbit model,5×106 mL-1 was transplanted into degenerated intervertebral disc. At the end of 1st,2nd,4th,8th week,im-munofluorescence and confocal laser scanning microscope were used to evaluate the expression and regularity of EGF,PDGF, HIF-1α,type II collagen in MSCs. Results At 2nd week,the expression of EGF and PDGF in MSCs hit the peak,which were (28.92±5.66)%and (40.77±8.13)%respectively,and then descended;the HIF-1αand typeⅡcollagen rose slowly,and peaked at the 8th week,which were(55.35±14.06)%and (37.23±9.53)%respectively,and the rising velocity was fastest during 2nd to 4th week. Conclusion The trophic effects play a major part in the MSCs in early(2nd week) period,then self differentiated effects play a ma-jor part in later regeneration(4 weeks later).