临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2014年
20期
1663-1665
,共3页
刘占东%吴岩珏%王得新%薛启蓂%张拥波%刘天会%李任%张健%杨伊姝%谢敏豪
劉佔東%吳巖玨%王得新%薛啟蓂%張擁波%劉天會%李任%張健%楊伊姝%謝敏豪
류점동%오암각%왕득신%설계명%장옹파%류천회%리임%장건%양이주%사민호
大鼠%中枢性疲劳%训练%色氨酸%亮氨酸%支链氨基酸
大鼠%中樞性疲勞%訓練%色氨痠%亮氨痠%支鏈氨基痠
대서%중추성피로%훈련%색안산%량안산%지련안기산
Rats%Central fatigue%Exercise%Tryptophan%Leucine%Branched-Chain Amino Acids
目的：通过对于慢性运动性中枢疲劳模型大鼠血清色氨酸及亮氨酸含量测定，探讨色氨酸及支链氨基酸在慢性运动性中枢疲劳调控机制方面的变化规律。方法建立大鼠慢性运动性中枢疲劳模型；应用高效液相色谱-质谱的研究方法对大鼠血清色氨酸及亮氨酸进行测定。结果与正常饲养大鼠比较发现，奔跑条件下运动性中枢疲劳大鼠血清中色氨酸水平明显降低，差异有显著性（ P <0.05）；亮氨酸的水平无统计学差异（ P >0.05）；亮氨酸/色氨酸明显升高，差异有显著性（ P <0.05）。结论慢性运动性中枢疲劳时大鼠血中色氨酸表现为明显下降的趋势，支链氨基酸亮氨酸与其比值明显升高可能进一步抑制其进入中枢，调控中枢性疲劳的发生和发展，该变化可能是机体为适应慢性运动性中枢疲劳状态的一种自我调控反应。
目的：通過對于慢性運動性中樞疲勞模型大鼠血清色氨痠及亮氨痠含量測定，探討色氨痠及支鏈氨基痠在慢性運動性中樞疲勞調控機製方麵的變化規律。方法建立大鼠慢性運動性中樞疲勞模型；應用高效液相色譜-質譜的研究方法對大鼠血清色氨痠及亮氨痠進行測定。結果與正常飼養大鼠比較髮現，奔跑條件下運動性中樞疲勞大鼠血清中色氨痠水平明顯降低，差異有顯著性（ P <0.05）；亮氨痠的水平無統計學差異（ P >0.05）；亮氨痠/色氨痠明顯升高，差異有顯著性（ P <0.05）。結論慢性運動性中樞疲勞時大鼠血中色氨痠錶現為明顯下降的趨勢，支鏈氨基痠亮氨痠與其比值明顯升高可能進一步抑製其進入中樞，調控中樞性疲勞的髮生和髮展，該變化可能是機體為適應慢性運動性中樞疲勞狀態的一種自我調控反應。
목적：통과대우만성운동성중추피로모형대서혈청색안산급량안산함량측정，탐토색안산급지련안기산재만성운동성중추피로조공궤제방면적변화규률。방법건립대서만성운동성중추피로모형；응용고효액상색보-질보적연구방법대대서혈청색안산급량안산진행측정。결과여정상사양대서비교발현，분포조건하운동성중추피로대서혈청중색안산수평명현강저，차이유현저성（ P <0.05）；량안산적수평무통계학차이（ P >0.05）；량안산/색안산명현승고，차이유현저성（ P <0.05）。결론만성운동성중추피로시대서혈중색안산표현위명현하강적추세，지련안기산량안산여기비치명현승고가능진일보억제기진입중추，조공중추성피로적발생화발전，해변화가능시궤체위괄응만성운동성중추피로상태적일충자아조공반응。
Objective The aim of this paper is to analyze mechanisms of motion regulation of tryptophan and branched-chain in chronic ex-ercise central fatigue,through the analysis of tryptophan and leucine in serum in ratˊs model of chronic exercise central fatigue. Methods To set up the ratˊs model of chronic exercise central fatigue. To test the tryptophan and leucine in Ratsˊserum with HPLC-MS. Results Compared with the control rats,the level of tryptophan in exercise central fatigue rats reduces sharply and there is a significant difference( P <0. 05). There is no sta-tistical difference in the level of leucine. Leu/Trp rises sharply with significant difference( P <0. 05). Conclusion There is a clear trend of decli-ning in the level of tryptophan in the blood of rats under training with chronic exercise central fatigue. Because of the rising level of the ratio between leucine and tryptophan in branched-chain amino acids. It refines tryptophan to entering the central nerves system,regulating the occurring and de-velopment of the central fatigue. This alternation is possibly a self-regulation reaction in order to adapt to chronic exercise central fatigue.
