CAJ | 학술논문

目的：分析全血γ-干扰素释放试验对结核病辅助诊断价值。方法随机选取我院收治的肺部疾病患者436例，分为4组，第1组为痰涂片抗酸杆菌阳性的肺结核45例；第2组为痰涂片阴性的肺结核240例；第3组为经临床病理学或排除肺结核的非结核肺部疾病151例；第4组为对健康对照20例。用酶联免疫吸附试验（elisa）分别检测4组受试者血清γ-干扰素水平。结果第1组和第2组活动性肺结核患者血清γ-干扰素水平显著高于第3组和第4组非结核肺部疾病组和对照组，有统计学差异（P＜0.0083），敏感性分别为78.3%和83.3%，而第1组和第2组活动性肺结核患者之间血清γ-干扰素水平无统计学差异（P＞0.0083），第3组和第4组非结核肺部疾病组和对照组差异无统计学意义（P＞0.0083）。结论全血γ-干扰素释放试验可作为肺结核的重要辅助诊断技术，值得临床应用和推广。
목적：분석전혈γ-간우소석방시험대결핵병보조진단개치。방법수궤선취아원수치적폐부질병환자436례，분위4조，제1조위담도편항산간균양성적폐결핵45례；제2조위담도편음성적폐결핵240례；제3조위경림상병이학혹배제폐결핵적비결핵폐부질병151례；제4조위대건강대조20례。용매련면역흡부시험（elisa）분별검측4조수시자혈청γ-간우소수평。결과제1조화제2조활동성폐결핵환자혈청γ-간우소수평현저고우제3조화제4조비결핵폐부질병조화대조조，유통계학차이（P＜0.0083），민감성분별위78.3%화83.3%，이제1조화제2조활동성폐결핵환자지간혈청γ-간우소수평무통계학차이（P＞0.0083），제3조화제4조비결핵폐부질병조화대조조차이무통계학의의（P＞0.0083）。결론전혈γ-간우소석방시험가작위폐결핵적중요보조진단기술，치득림상응용화추엄。
Objective to analyze diagnostic value of the whole blood gamma interferon release test on lung tuberculosis. Methods 436 patients were enrolled in the study, which were divided into four groups, one group included 45 patients of pulmonary tuberculosis with smear positive, the second group included 240 cases of pulmonary tuberculosis with smear negative, and the third group included 151 patients identiifed with other pulmonary disease besides tuberculosis. The fourth group enrolled 20 healthy volunteers. enzyme linked immunosorbent assay (elisa) method was used to detect serum level of the gamma interferon in the subjects of four groups. Results The level of serum gamma interferon of patients in the ifrst and second group with active tuberculosis were signiifcantly higher than that in non tuberculosis group and the control group, with signiifcant differences (P<0.0083), with diagnostic sensitivity of 78.3%and 83.3%, respectively. there were no statistically signiifcant differences in the level of serum gamma interferon of patients between the ifrst and second groups, and also between the third and fourth groups. Conclusion the whole blood gamma interferon release test could be used as important method in tuberculosis diagnosis, and it deserves to be applied in clinical promotion.