CAJ | 학술논문

目的：探讨FUT4和LeY在子宫内膜异位症患者中的表达情况及其临床价值。方法选择2010年3月~2012年6月辽宁省大连市妇幼保健院(以下简称“我院”)妇产科经腹腔镜手术证实为子宫内膜异位症的70例患者，将患者分为在位内膜组和异位内膜组，每组各35例。同时选择同期我院行手术治疗的单纯子宫肌瘤患者35例为对照组。采用免疫组化、RT-PCR检测三组患者子宫内膜组织中FUT4和LeY的表达情况，并对其变化的意义进行评价和分析。结果①与对照组比较，在位内膜组和异位内膜组患者的FUT4阳性表达率均明显提高，差异有统计学意义(P<0.05)，异位内膜组FUT4的阳性表达率高于在位内膜组(P<0.05)；②与对照组比较，在位内膜组和异位内膜组患者的LeY的阳性表达率均明显提高，差异有统计学意义(P<0.05)，异位内膜组LeY的阳性表达率高于在位内膜组，差异有统计学意义(P<0.05)；③与对照组比较，FUT4在异位内膜组和在位内膜组中的阳性表达率明显高于对照组，差异有统计学意义(P<0.05)，异位内膜组FUT4的阳性表达率高于在位内膜组(P<0.05)。结论 FUT4和LeY不仅在子宫内膜异位症的形成和发展过程中发挥着重要作用，其在胚胎黏附、植入过程中也起着重要作用，而FUT4是LeY合成的关键酶，当其表达量高于某一临界值时，会诱发子宫内膜异位症，影响胚胎的正常着床，从而进一步导致不孕。
목적：탐토FUT4화LeY재자궁내막이위증환자중적표체정황급기림상개치。방법선택2010년3월~2012년6월요녕성대련시부유보건원(이하간칭“아원”)부산과경복강경수술증실위자궁내막이위증적70례환자，장환자분위재위내막조화이위내막조，매조각35례。동시선택동기아원행수술치료적단순자궁기류환자35례위대조조。채용면역조화、RT-PCR검측삼조환자자궁내막조직중FUT4화LeY적표체정황，병대기변화적의의진행평개화분석。결과①여대조조비교，재위내막조화이위내막조환자적FUT4양성표체솔균명현제고，차이유통계학의의(P<0.05)，이위내막조FUT4적양성표체솔고우재위내막조(P<0.05)；②여대조조비교，재위내막조화이위내막조환자적LeY적양성표체솔균명현제고，차이유통계학의의(P<0.05)，이위내막조LeY적양성표체솔고우재위내막조，차이유통계학의의(P<0.05)；③여대조조비교，FUT4재이위내막조화재위내막조중적양성표체솔명현고우대조조，차이유통계학의의(P<0.05)，이위내막조FUT4적양성표체솔고우재위내막조(P<0.05)。결론 FUT4화LeY불부재자궁내막이위증적형성화발전과정중발휘착중요작용，기재배태점부、식입과정중야기착중요작용，이FUT4시LeY합성적관건매，당기표체량고우모일림계치시，회유발자궁내막이위증，영향배태적정상착상，종이진일보도치불잉。
Objective To explore the expression of LeY and FUT4 in patients with endometriosis and its clinical value. Methods 70 patients with endometriosis confirmed by laparoscopic surgery from March 2010 to June 2012 in Depart-ment of Gynecology and Obstetrics, Maternal and Child Health Hospital of Dalian City (“our hospital” for short), and these patients were divided into eutopic endometrial group and ectopic endometrial group, with 35 patients in each group. 35 patients with uterine fibroids alone in our hospital during the same period were also selected as the control group. Immunohistochemistry and RT-PCR were used to detect the expression of FUT4 and LeY in endometrial tissues of three groups, the significance of their changes were evaluated and analyzed. Results ①Compared with the control group, the positive expression rate of FUT4 in eutopic endometrial group and ectopic endometrium group were signifi-cantly improved, and the difference was statistically significant (P<0.05), and the positive expression rate of FUT4 in ectopic endometrial group was higher than that in the eutopic endometrial group, the difference was statistically signifi-cant (P< 0.05). ②Compared with the control group, positive expression rate of LeY in the eutopic endom etrial group and ectopic endometrium group were significantly improved, and the difference was statistically significant (P< 0.05), positive expression rate of LeY in the ectopic endometrial group was higher than that in the eutopic endometrial group, the difference was statistically significant (P < 0.05).③Compared with the control group, positive expression rate of FUT4 in the ectopic endometrial group and eu-topic endometrial group was significantly higher, and the difference was statistically significant (P < 0.05), while the positive expression rate of FUT4 in the ectopic endometrial group was higher than that in the eutopic endometrial group, the difference was statistically significant (P< 0.05). Conclusion FUT4 and LeY not only plays an important role in the formation and development of endometriosis but also in embryonic adhesion and implantation process, espe-cially FUT4 is a key enzyme in the synthesis of LeY, while if a certain threshold is reached with its high expression, it will induce endometriosis, affecting the normal implantation of the embryo, which further leads to infertility.