CAJ | 학술논문

目的：探讨五味子甲素（SchA ）缓解1-甲基-4-苯基吡啶离子（M PP＋）诱导S H-SY5Y细胞损伤的可能机制。方法实验分为5组，空白对照组，加入无血清培养基进行培养；模型组（M PP＋进行诱导）；SchA组；SchA与M PP＋共同处理的SchA组，其中SchA终浓度分别为1、3、5μmol/L ，M PP＋终浓度为1 mmol/L。NO检测试剂盒检测各组细胞 NO含量，Western blot检测细胞总Akt、p-Akt的蛋白表达情况。结果与对照组相比，1 mmol/L MMP＋诱导后，SH-SY5Y细胞NO含量明显增高（P＜0．05），经5μmol/L SchA及MPP＋共同处理的SH-SY5Y细胞，NO的含量明显降低。各组总Akt相对表达水平没有明显变化（P＞0．05）；模型组细胞p-Akt相对表达水平较对照组减少，SchA组细胞p-Akt蛋白相对表达水平随SchA浓度的升高而升高，且与模型组比较差异都有统计学意义（P＜0．05）。结论 SchA缓解MPP＋诱导的SH-SY5Y细胞损伤可能与影响NO含量及p-Akt蛋白表达有关。
목적：탐토오미자갑소（SchA ）완해1-갑기-4-분기필정리자（M PP＋）유도S H-SY5Y세포손상적가능궤제。방법실험분위5조，공백대조조，가입무혈청배양기진행배양；모형조（M PP＋진행유도）；SchA조；SchA여M PP＋공동처리적SchA조，기중SchA종농도분별위1、3、5μmol/L ，M PP＋종농도위1 mmol/L。NO검측시제합검측각조세포 NO함량，Western blot검측세포총Akt、p-Akt적단백표체정황。결과여대조조상비，1 mmol/L MMP＋유도후，SH-SY5Y세포NO함량명현증고（P＜0．05），경5μmol/L SchA급MPP＋공동처리적SH-SY5Y세포，NO적함량명현강저。각조총Akt상대표체수평몰유명현변화（P＞0．05）；모형조세포p-Akt상대표체수평교대조조감소，SchA조세포p-Akt단백상대표체수평수SchA농도적승고이승고，차여모형조비교차이도유통계학의의（P＜0．05）。결론 SchA완해MPP＋유도적SH-SY5Y세포손상가능여영향NO함량급p-Akt단백표체유관。
Objective To explore the possible mechanism of SchA ,which decreases MPP+induce SH-SY5Y cell damage .Meth-ods Cultured cells were divided into 5 groups ,one as control group ,cultured by free-blood serum media;the other 4 groups were treated with different concentrations of SchA(1 ,3 ,5 μmol/L) and MPP+ (1 mmol/L) for 48 h named model group ,1 ,3 ,5 μmol/L SchA group respetivly .The content of nitric oxide(NO) were measured by NO kit ;The expression levels of total Akt and p-Akt proteins were detected by Western blot .Results Compared with the control group ,the content of NO in group significantly in-creased after MPP+stimulating(P<0 .05);compared to the control group ,the content of NO in 5μmol/L SchA group significantly decreased(P<0 .05) .The expression levels of total Akt in all groups had no significant difference(P>0 .05) .The expression levels of p-Akt in model group significantly lowered ,while SchA(1、3、5 μmol/L) significantly increased the expression levels of p-Akt in comparision with cells in model group .Conclusion Decreasing MPP+ induced SH-SY5Y cell damage of SchA may be related to the content of NO and p-Akt expression .