CAJ | 학술논문

目的：研究外用重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）和纳米银敷料联合外用对深Ⅱ°烫伤创面愈合过程炎性反应的影响。方法用Wistar大鼠建立深Ⅱ°烫伤模型，分为A、B、C、D 4组，A组30例：凡士林纱布覆盖；B组30例：纳米银敷料覆盖；C组30例：rhGM-CSF涂抹创面；D组30例：rhGM-CSF联合纳米银敷料覆盖，伤后第1、4、7、10、14、21天，观察创面炎性反应，细菌培养并计数，按照ELISA测定血清IL-2、IL-8水平。结果炎性反应由重至轻依次为A组、B组、C组、D组；A组、B组/C组、D组分别于伤后第4、10、14天出现细菌生长，D组与A、B、C组比较，B、C组与 A组比较，细菌生长数量少，且第10、14、21天差异有统计学意义（P＜0．05）；各组间IL-2、IL-8水平各时相点不同，D组最低，A组最高；IL-2水平，除伤后第1天各组间差异无统计学意义，第4天，B组与C组间差异无统计学（P＞0．05）外，其余各时相点各组间差异有统计学意义（P＜0．05）；IL-8水平，除第1天，A组与B组、B组与C组、D组与C组间差异无统计学意义（ P＞0．05）外，在各时相点各组间差异均有统计学意义（P＜0．05）。结论 rhGM-CSF和纳米银联合外用，减轻深Ⅱ°烫伤创面炎性反应，并且优于rhGM-CSF、纳米银单独应用。
목적：연구외용중조인립세포-거서세포집락자격인자（rhGM-CSF）화납미은부료연합외용대심Ⅱ°탕상창면유합과정염성반응적영향。방법용Wistar대서건립심Ⅱ°탕상모형，분위A、B、C、D 4조，A조30례：범사림사포복개；B조30례：납미은부료복개；C조30례：rhGM-CSF도말창면；D조30례：rhGM-CSF연합납미은부료복개，상후제1、4、7、10、14、21천，관찰창면염성반응，세균배양병계수，안조ELISA측정혈청IL-2、IL-8수평。결과염성반응유중지경의차위A조、B조、C조、D조；A조、B조/C조、D조분별우상후제4、10、14천출현세균생장，D조여A、B、C조비교，B、C조여 A조비교，세균생장수량소，차제10、14、21천차이유통계학의의（P＜0．05）；각조간IL-2、IL-8수평각시상점불동，D조최저，A조최고；IL-2수평，제상후제1천각조간차이무통계학의의，제4천，B조여C조간차이무통계학（P＞0．05）외，기여각시상점각조간차이유통계학의의（P＜0．05）；IL-8수평，제제1천，A조여B조、B조여C조、D조여C조간차이무통계학의의（ P＞0．05）외，재각시상점각조간차이균유통계학의의（P＜0．05）。결론 rhGM-CSF화납미은연합외용，감경심Ⅱ°탕상창면염성반응，병차우우rhGM-CSF、납미은단독응용。
Objective To observe the effect of recombinant human granulocyte/macrophage colonystimulating factor (rhGM-CSF) combined with nano-silver as a treatment on deep burn degreen Ⅱ about inflammation .Methods The burn model was built with Wistar rats .They were randomly divided into four groups ,petrolatum treatment (group A ,n= 30) ,nano-silver treatment (group B ,n=30) ,rhGM-CSF treatment(group C ,n=30) ,and rhGM-CSF combined with nano-silver treatment(group D ,n=30) . observation the inflammatory reaction ,and culture bacteria on wound of the four groups at 1st ,4th ,7th ,10th ,14th ,21th day after treatment were made .The level of IL-2 and IL-8 were measured in serums with ELISA .Results The inflammatory reaction:group A>group B>group C>group D ;Bacterias were observed in group A ,group B/C and group D at 4th ,10th ,14th day respectively af-ter treatment .The number of bacterial growed in group D was less than in group A ,group B and group C .The numbers of bacterial growed in group B and group C were less than in group A .And after 10 ,14 ,21 days treatment ,there was significantly statistical difference(P<0 .05) .There was difference among groups in the levele of IL-2 and IL-8 ,which were the lowest in group D and the highest in group A .The level of IL-2 has no significantly statistical difference between every groupat 1st day and between B and C group at 4th day(P>0 .05) .After other days treatment ,there was significantly statistical difference(P<0 .05) .There was signifi-cantly statistical difference in every group at each day of the IL-8 levele except A and B group ,B and C group ,C and D group at 1st day (P<0 .05) .Conclusion rhGM-CSF combined with nano-silver treatment could alleviate inflammatory reaction ,and be better than rhGM-CSF or nano-silver alone .