CAJ | 학술논문

目的：建立根管-根尖周组织复合体体外模型，以此探索感染根管形成根尖周炎及根尖生物膜的可能机制。方法将因正畸减数拔除的健康单根前磨牙密封于盛有LB固体培养基的无菌小瓶内，使牙根的根尖1/3置于培养基中，制备成根管-根尖周复合体体外模型25个。建模后1 d抽取5个模型通过PCR技术检测根尖周组织有无细菌。将余下的20个模型分为对照组（n＝10）及实验组（n＝10），实验组作开髓处理，对照组不做任何处理，自然放置于空气中，经21 d检测根管内和根尖周有无细菌，及终点显色法检测根尖周内毒素浓度。结果第21天，实验组根管内检测到细菌，对照组根管内未检测到细菌，而所有模型根尖周均检测到细菌；对照组根尖周内毒素含量平均为（8．913±0．614）EU/mL ，实验组为（10．525±0．981）EU/mL ，两组比较差异有统计学意义（P＜0．01）。结论通过上述方法成功建立根管-根尖周复合体体外模型。未对根管做处理时，感染根管内的细菌不会到达根尖周，而感染根管内的细菌首先是通过分泌的内毒素等致病因子到达根尖周引起根尖周炎。
목적：건립근관-근첨주조직복합체체외모형，이차탐색감염근관형성근첨주염급근첨생물막적가능궤제。방법장인정기감수발제적건강단근전마아밀봉우성유LB고체배양기적무균소병내，사아근적근첨1/3치우배양기중，제비성근관-근첨주복합체체외모형25개。건모후1 d추취5개모형통과PCR기술검측근첨주조직유무세균。장여하적20개모형분위대조조（n＝10）급실험조（n＝10），실험조작개수처리，대조조불주임하처리，자연방치우공기중，경21 d검측근관내화근첨주유무세균，급종점현색법검측근첨주내독소농도。결과제21천，실험조근관내검측도세균，대조조근관내미검측도세균，이소유모형근첨주균검측도세균；대조조근첨주내독소함량평균위（8．913±0．614）EU/mL ，실험조위（10．525±0．981）EU/mL ，량조비교차이유통계학의의（P＜0．01）。결론통과상술방법성공건립근관-근첨주복합체체외모형。미대근관주처리시，감염근관내적세균불회도체근첨주，이감염근관내적세균수선시통과분비적내독소등치병인자도체근첨주인기근첨주염。
Objective To establish an in-vitro root canal-apical complex model for studying the mechanisms of the infected root canal to apical periodontitis and periapical biofilm .Methods Single rooted premolar extracted for orthodontic ,was sealed in sterile vial containing LB solid medium ,and the culture medium covered apical thirds .Totally 25 root canal-apical complex models were prepared .Five models were randomly selected for the bacteria detection in periapical by PCR at 1st day .The remained 20 models were randomly subjected to a control group(n=10) and experimental group(n=10) .Extracted teeth were opened in experimental group and control group with no treatment .All models were exposed in air .At 21st day ,bacteria were detected through PCR in root canal and apical;endotoxin content in apical was assayed by chromogenic end-point limulus test .Results In apical ,bacteria was not found in all groups ,but not for the experimental group .The mean endotoxin content was (8 .913 ± 0 .614)EU/mL in control group and (10 .525 ± 0 .981)EU/mL in experimental group .The endotoxin content was increased significantly in experimental group ,when compared with control group(P<0 .01) .Conclusion Root canal-apical complex was established in vitro through this method .Bac-teria was not easy to reach the apical when the infected root canal was not disturbed .Bacteria in the infected root canal caused apical periodontitis through the secretion of virulence factors such as endotoxin .