CAJ | 학술논문

目的：了解贵州地区丙型肝炎病毒（HCV ）基因型的分布特点及其与感染途径、性别、年龄因素的关系。方法在198份抗HCV抗体和HCV RNA均阳性的血清标本中，分别提取HCV RNA ，通过逆转录巢式PCR（RT nested-PCR）扩增C基因的羧基端至E1基因的氨基端长度为474 bp的片段，测定其核苷酸序列，与GenBank中已知的 HCV序列进行系谱分析，确定HCV基因亚型。结果198例患者中，HCV 1a型4例（2．0％）；1b型例71例（35．9％）；2a型9例（4．6％）；3a型29例（14．7％），3b型47例（23．7％），6a型37例（18．7％），6d型1例（0．5％）。各基因型在男女性别分布比较，差异无统计学意义（χ2＝6．518， P＝0．885）；不同年龄人群 HCV基因型分布比较，差异有统计学意义（χ2＝49．056，P＝0．005）；不同感染途径人群 HCV基因型分布比较，差异有统计学意义（χ2＝99．245，P＝0．005）。结论贵州地区 HCV 基因型主要为1b型，其次为3b、6a、3a型，同时还存在1a、2a和6d等基因型。HCV基因型与其感染途径有关，基因亚型呈现多样性。
목적：료해귀주지구병형간염병독（HCV ）기인형적분포특점급기여감염도경、성별、년령인소적관계。방법재198빈항HCV항체화HCV RNA균양성적혈청표본중，분별제취HCV RNA ，통과역전록소식PCR（RT nested-PCR）확증C기인적최기단지E1기인적안기단장도위474 bp적편단，측정기핵감산서렬，여GenBank중이지적 HCV서렬진행계보분석，학정HCV기인아형。결과198례환자중，HCV 1a형4례（2．0％）；1b형례71례（35．9％）；2a형9례（4．6％）；3a형29례（14．7％），3b형47례（23．7％），6a형37례（18．7％），6d형1례（0．5％）。각기인형재남녀성별분포비교，차이무통계학의의（χ2＝6．518， P＝0．885）；불동년령인군 HCV기인형분포비교，차이유통계학의의（χ2＝49．056，P＝0．005）；불동감염도경인군 HCV기인형분포비교，차이유통계학의의（χ2＝99．245，P＝0．005）。결론귀주지구 HCV 기인형주요위1b형，기차위3b、6a、3a형，동시환존재1a、2a화6d등기인형。HCV기인형여기감염도경유관，기인아형정현다양성。
Objective To study the distribution of genotypes of hepatitis C virus in Guizhou and its relationship between infec-tious route of genotype and age ,gender was analyzed .Methods Serum specimens in this study were obtained from 198 patients , whose anti-HCV and HCV RNA were positive .A reverse transcriptase PCR(RT nested-PCR)assay using conserved primers de-duced from the core-envelope 1(C-E1)region of the hepatitis C virus(HCV)genome was employed to amplify a 474-nucleotide-long fragment .Phylogenetic analysis of the C-E1 sequences was conducted by direct sequencing of the RT-PCR products and alignment with published HCV subtypes in GenBank .Subtypes of the samples were determined by nucleotide sequencing followed by composi-tion of a phylogenetic tree .Results Among the 198 patients surveyed ,genotype 1a was detected in 4 cases(2 .0% ) ,genotype 1b in 71 cases(35 .9% ) ,genotype 2a in 9 cases(4 .6% ) ,genotype 3a in 29 cases(14 .7% ) ,genotype 3b in 47 cases(23 .7% ) ,genotype 6 a in 37 cases(18 .7% )and genotype 6d in 1 cases(0 .5% ) .Genotype distribution on gender had no statistical significance(P>0 .05) , and its distribution on people with different ages had statistical significance(P<0 .05) ,and its distribution on patients with different infectious routes was significantly different(P<0 .05) .Conclusion The major genotypes of HCV are 1b ,3b ,6a and 3a in Guizhou , and genotype 1a is predominant .Genotypes 1a ,2a and 6d exist too .Genotypes of patients infected with HCV are related to their in-fectious routes ,and the HCV genotypes are in a great variety .