CAJ | 학술논문

目的：研究γ氨基丁酸（GABA）对野百合碱（MCT ）诱导肺动脉高压大鼠的治疗作用，并检测血管内皮生长因子（VEGF）、基质金属蛋白酶-9（MMP-9）表达水平的变化。方法应用随机数字表法将30只雄性SD大鼠随机分3组，分别为正常对照组（对照组）、MCT模型组（模型组）、γ氨基丁酸干预组（干预组），每组10只。观测平均右心室压（mRVP），计算右心室肥厚指数（RVHI），测定肺小动脉管壁厚度与动脉外直径的百分比（WT％）及管壁面积与血管总面积的百分比（WA％），免疫组织化学染色法观察MMP-9、实时荧光定量PCR（FQ-PCR）检测VEGF mRNA在肺组织中的表达。结果干预组mRVP、RVHI、WT％、WA％、MMP-9蛋白和VEGF mRNA的表达水平明显高于对照组（P＜0．05）；而低于模型组（P＜0．05）。结论 GABA可抑制野百合碱诱导的肺动脉高压模型大鼠肺组织VEGF mRNA和MMP-9蛋白的表达，对肺动脉高压有治疗作用。
목적：연구γ안기정산（GABA）대야백합감（MCT ）유도폐동맥고압대서적치료작용，병검측혈관내피생장인자（VEGF）、기질금속단백매-9（MMP-9）표체수평적변화。방법응용수궤수자표법장30지웅성SD대서수궤분3조，분별위정상대조조（대조조）、MCT모형조（모형조）、γ안기정산간예조（간예조），매조10지。관측평균우심실압（mRVP），계산우심실비후지수（RVHI），측정폐소동맥관벽후도여동맥외직경적백분비（WT％）급관벽면적여혈관총면적적백분비（WA％），면역조직화학염색법관찰MMP-9、실시형광정량PCR（FQ-PCR）검측VEGF mRNA재폐조직중적표체。결과간예조mRVP、RVHI、WT％、WA％、MMP-9단백화VEGF mRNA적표체수평명현고우대조조（P＜0．05）；이저우모형조（P＜0．05）。결론 GABA가억제야백합감유도적폐동맥고압모형대서폐조직VEGF mRNA화MMP-9단백적표체，대폐동맥고압유치료작용。
Objective To study the therapeutical effect of γ-aminobutyric acid on monocrotaline(MCT )induced pulmonary hy-pertension rats ,and to elucidate the expression of VEGF and MMP-9 .Methods Thirty SD rats were randomly divided into 3 groups :a normal control group(control group) ,a MCT-induced pulmonary hypertension group(model control group) ,and anγ-ami-nobutyric acid treatment group(treatment group) .The mean right ventricular pressure(mRVP)were detected ,the right ventricular hypertrophy index(RVHI)were measured ,WT% and WA% were evaluated ,and the expression of VEGF mRNA in the lung tissue and MMP-9 were detected wtih FQ-PCR and immunohistochemical staining method respectively .Results mRVP ,RVHl ,WT% , WA% and the expression of MMP-9 and VEGF mRNA of treatment group were lower than those in the model control group(P<0 .05) ,but higher than the control group(P<0 .05) .Conclusion GABA has a therapeutic effect on pulmonary hypertension rats through regulating the expression of VEGF mRNA and MMP-9 protein .