CAJ | 학술논문

目的：观察辛伐他汀对高糖损伤血管内皮细胞的保护作用，并探讨其作用的机制。方法实验分为4组：对照组（健康体检者，A组），高血糖组（B组，33．3 mmol/L葡萄糖），高血糖＋低浓度辛伐他汀组（C组，33．3 mmol/L葡萄糖＋1．0μmol/L辛伐他汀），高血糖＋高浓度辛伐他汀组（D组，33．3mmol/L葡萄糖＋10．0μmol/L辛伐他汀）。细胞增殖使用CCK-8实验检测，细胞凋亡使用 TUNEL 检测法，蛋白表达使用 Western blot 方法检测，基因检测采用实时荧光定量 PCR（real time PCR）。结果 B、C、D组的内皮细胞存活率分别为（42．5±6．4）％、（58．6±7．8％）和（71．3±11．7）％，各组之间差异有统计学意义（ P＜0．05）。A、B、C、D组内皮细胞的凋亡率分别为（1．8±0．6）％、（45．8±8．9）％、（22．7±6．4）％和（12．6±4．2）％，各组之间差异有统计学意义（ P＜0．05）。B组（0．13±0．03）内皮细胞Bcl-2蛋白的表达明显低于A组（1．02±0．16），C组（0．28±0．04）明显高于B组，D组（0．68±0．11）又明显高于C组（P＜0．05，P＜0．01）。B组内皮细胞bax基因的表达明显高于A组，C组明显低于B组， D组又明显低于C组（ P＜0．05，P＜0．01）。结论辛伐他汀可以明显抑制高糖诱导的内皮细胞凋亡，上调Bcl-2蛋白表达和下调bax基因表达可能是其主要的作用机制。
목적：관찰신벌타정대고당손상혈관내피세포적보호작용，병탐토기작용적궤제。방법실험분위4조：대조조（건강체검자，A조），고혈당조（B조，33．3 mmol/L포도당），고혈당＋저농도신벌타정조（C조，33．3 mmol/L포도당＋1．0μmol/L신벌타정），고혈당＋고농도신벌타정조（D조，33．3mmol/L포도당＋10．0μmol/L신벌타정）。세포증식사용CCK-8실험검측，세포조망사용 TUNEL 검측법，단백표체사용 Western blot 방법검측，기인검측채용실시형광정량 PCR（real time PCR）。결과 B、C、D조적내피세포존활솔분별위（42．5±6．4）％、（58．6±7．8％）화（71．3±11．7）％，각조지간차이유통계학의의（ P＜0．05）。A、B、C、D조내피세포적조망솔분별위（1．8±0．6）％、（45．8±8．9）％、（22．7±6．4）％화（12．6±4．2）％，각조지간차이유통계학의의（ P＜0．05）。B조（0．13±0．03）내피세포Bcl-2단백적표체명현저우A조（1．02±0．16），C조（0．28±0．04）명현고우B조，D조（0．68±0．11）우명현고우C조（P＜0．05，P＜0．01）。B조내피세포bax기인적표체명현고우A조，C조명현저우B조， D조우명현저우C조（ P＜0．05，P＜0．01）。결론신벌타정가이명현억제고당유도적내피세포조망，상조Bcl-2단백표체화하조bax기인표체가능시기주요적작용궤제。
Objective To study the effect of simvastatin on high glucose induced vascular endothelial cells injury and explore its action mechanism .Methods Endothelial cells were devided into four group:control group(A) ,high glucose(33 .3 mmol/L)group (B) ,high glucose(33 .3 mmol/L)+simvastatin(1 .0 μmol/L)group(C) ,and high glucose(33 .3 mmol/L)+simvastatin(10 .0 μmol/L)group(D) .The inhibition of cells was detected by CCK-8 assay .The apoptosis was determined by TUNEL assay .The protein ex-pression was detected by Western blot analysis .Results The cells survival rates were (42 .5 ± 6 .4% ) ,(58 .6 ± 7 .8% ) ,and (71 .3 ± 11 .7% ) in B ,C ,and D group respectively .There was significant difference among the three groups(P<0 .05) .The per-cents of apoptosis were (1 .8 ± 0 .6% ) ,(45 .8 ± 8 .9% ) ,(22 .7 ± 6 .4% ) and (12 .6 ± 4 .2% ) in A ,B ,C and D group ,respectively . There was significant difference among the four groups(P<0 .05) .The protein expression of Bcl-2 was lower in B group(0 .13 ± 0 . 03)than that in A group(1 .02 ± 0 .16) .The Bcl-2 expression was higher in C group(0 .28 ± 0 .04)than that in B group ,and in D group(0 .68 ± 0 .11)than that in C group(P<0 .05 ,P<0 .01) .The gene expression of bax was higher in B group than that in A group .The bax expression was lower in C group than that in B group ,and the bax expression was lower in D group than that in C groups(P<0 .05 ,P<0 .01) .Conclusion Simvastatin can inhibit high glucose induced vascular endothelial cells apoptosis .Upregu-lation of Bcl-2 and downregulation of bax may be its action mechanism .