CAJ | 학술논문

目的：研究Toll样受体2（TLR2）和TLR4在光滑念珠菌肺部感染大鼠中的作用。方法将36只大鼠分为3组，每组12只：对照组（A组）、未免疫抑制肺部光滑念珠菌感染组（B组）及免疫抑制肺部光滑念珠菌感染组（C组）。每组分别在造模成功后第1、3天各处死大鼠6只，观察肺组织病理变化，用逆转录PCR（RT-PCR）法检测肺组织 TLR2、TLR4 mRNA的表达、酶联免疫吸附实验（ELISA）法测定TNF-α蛋白水平。结果 A组肺组织正常；B组部分肺泡塌陷、炎症细胞浸润，未见孢子或菌丝；C组大部分肺泡塌陷，部分扩张，大量炎症细胞浸润、并有孢子菌丝聚积。第1、3天 TLR2 mRNA和 TNF-α蛋白表达水平C组明显高于其他两组（P＜0．01），B组高于A组（P＜0．05）；C组第3天 TLR2、TLR4 mRNA ，TNF-α表达水平高于第1天（P＜0．05，P＜0．01），且TLR4表达高于A组（P＜0．05）。结论 TLR2和 TNF-α可能参与光滑念珠菌肺部感染的发生发展，TLR4可能起协同作用。
목적：연구Toll양수체2（TLR2）화TLR4재광활념주균폐부감염대서중적작용。방법장36지대서분위3조，매조12지：대조조（A조）、미면역억제폐부광활념주균감염조（B조）급면역억제폐부광활념주균감염조（C조）。매조분별재조모성공후제1、3천각처사대서6지，관찰폐조직병리변화，용역전록PCR（RT-PCR）법검측폐조직 TLR2、TLR4 mRNA적표체、매련면역흡부실험（ELISA）법측정TNF-α단백수평。결과 A조폐조직정상；B조부분폐포탑함、염증세포침윤，미견포자혹균사；C조대부분폐포탑함，부분확장，대량염증세포침윤、병유포자균사취적。제1、3천 TLR2 mRNA화 TNF-α단백표체수평C조명현고우기타량조（P＜0．01），B조고우A조（P＜0．05）；C조제3천 TLR2、TLR4 mRNA ，TNF-α표체수평고우제1천（P＜0．05，P＜0．01），차TLR4표체고우A조（P＜0．05）。결론 TLR2화 TNF-α가능삼여광활념주균폐부감염적발생발전，TLR4가능기협동작용。
Objective To study the roles of Toll-like receptor 2(TLR2)and Toll-like receptor 4(TLR4)in the progress of pulmo-nary infection with candida glabrata in experimental mice .Methods The mice were divided into three groups ,the control group (group A) ,mice infected with candida glabrata(group B)and immunosuppressive mice infected with candida glabrata(group C) .6 mice in each group were killed at 1st and 3rd day after the success in pulmonary infection with candida glabrata .The lung tissues from each group were collected for pathological analysis and PT-PCR to detect the expression level of TLR2 and TLR4 .Protein TNF alpha(TNF-α)level was measured by ELISA method .Results The pathological analysis showed the structure was normal and there was no inflammatory reaction in lungs in group A .The infiltration of inflammatory cells ,weak injuries but no conidia or hyphae in lungs were found in group B .Most of the alveolar collapse and severe damage ,part of the expansion ,a large number of in-flammatory cells infiltration ,accompanying with conidia and hyphae accumulation were observed in group C .The expression levels of TLR2 mRNA and TNF-αprotein in mice lungs at the 1st day and the 3rd day in group C were significantly higher than in group A and B(P<0 .05) ,while group B was higher than group A(P<0 .05);The expression levels of TLR2 ,TLR4 mRNA and TNF-αprotein in group C at the 3rd day was higher than the 1st day(P<0 .01) ,meanwhile ,the expression of TLR4 was higher than in group A(P<0 .05) .Conclusion TNF-αand TLR2 expression maybe involve in the infection and process of candida glabrata ,and TLR4 may play a synergistic effect .