护理研究
護理研究
호리연구
NURSING RESEARCH
2014年
31期
3867-3870
,共4页
宫颈癌%缺氧%H eLa 细胞%细胞干预%细胞存活
宮頸癌%缺氧%H eLa 細胞%細胞榦預%細胞存活
궁경암%결양%H eLa 세포%세포간예%세포존활
cervical cancer%hypoxia%HeLa cell%cell intervention%cell sur-vival
[目的]研究缺氧护理干预对人宫颈癌 HeLa 细胞周期和存活等的影响,以期为临床肿瘤护理新措施的制定提供依据。[方法] HeLa 细胞按常规用DMEM高糖培养基培养；制备1000μM的二氯化钴储存液后,分别构建50μM、100μM及200μM的二氯化钴浓度模拟轻度缺氧、中度缺氧及重度缺氧的环境,37℃孵育24 h后按流式细胞检测程序进行细胞周期和存活率测定及克隆形成能力测定。[结果]重度缺氧能引起 HeLa 细胞处于 G1期的细胞比例明显下降,处于 G2/M 期的细胞比例明显升高；重度缺氧还能造成 H eLa 细胞存活率降低；缺氧的严重程度与 H eLa 细胞克隆形成能力的抑制程度呈正相关。[结论]缺氧能严重影响 H eLa 细胞的生长和存活,护理干预可据此进行针对性的新措施制定和临床研究,以便进行更有效的宫颈癌病人个性化护理。
[目的]研究缺氧護理榦預對人宮頸癌 HeLa 細胞週期和存活等的影響,以期為臨床腫瘤護理新措施的製定提供依據。[方法] HeLa 細胞按常規用DMEM高糖培養基培養；製備1000μM的二氯化鈷儲存液後,分彆構建50μM、100μM及200μM的二氯化鈷濃度模擬輕度缺氧、中度缺氧及重度缺氧的環境,37℃孵育24 h後按流式細胞檢測程序進行細胞週期和存活率測定及剋隆形成能力測定。[結果]重度缺氧能引起 HeLa 細胞處于 G1期的細胞比例明顯下降,處于 G2/M 期的細胞比例明顯升高；重度缺氧還能造成 H eLa 細胞存活率降低；缺氧的嚴重程度與 H eLa 細胞剋隆形成能力的抑製程度呈正相關。[結論]缺氧能嚴重影響 H eLa 細胞的生長和存活,護理榦預可據此進行針對性的新措施製定和臨床研究,以便進行更有效的宮頸癌病人箇性化護理。
[목적]연구결양호리간예대인궁경암 HeLa 세포주기화존활등적영향,이기위림상종류호리신조시적제정제공의거。[방법] HeLa 세포안상규용DMEM고당배양기배양；제비1000μM적이록화고저존액후,분별구건50μM、100μM급200μM적이록화고농도모의경도결양、중도결양급중도결양적배경,37℃부육24 h후안류식세포검측정서진행세포주기화존활솔측정급극륭형성능력측정。[결과]중도결양능인기 HeLa 세포처우 G1기적세포비례명현하강,처우 G2/M 기적세포비례명현승고；중도결양환능조성 H eLa 세포존활솔강저；결양적엄중정도여 H eLa 세포극륭형성능력적억제정도정정상관。[결론]결양능엄중영향 H eLa 세포적생장화존활,호리간예가거차진행침대성적신조시제정화림상연구,이편진행경유효적궁경암병인개성화호리。
Obj ective:To study the influence of hypoxia nursing interven-tion on cycle and survival of HeLa cells in human cervical carcinoma,in order to provide the basis for formulating new measures of clinical oncol-ogy nursing.Methods:The HeLa cells were cultured in conventional DMEM high glucose medium;after 1 000μM Two cobalt chloride storage liquid were prepared,it respectively constructed two cobalt chloride con-centration of 50μM,100μM and 200μM to simulate mild hypoxia,mod-erate hypoxia and severe hypoxia environment.After incubating for 24 h at 3 7 ℃,then the cell cycle,cell survival rate and clone forming ability were measured according to the flow cytometry detection program.Re-sults:Severe hypoxia can obviously induce the percentage of G1 phase cells in HeLa cells decent,but the proportion of cells in G2/M phase in-creased significantly;severe hypoxia can lead to the survival rate of HeLa cell decent;the severity of hypoxia was positively correlated with the de-gree of inhibition of HeLa cell clone forming ability.Conclusion:Hypoxia can seriously affect the growth and survival of HeLa cells.According to this,the targeted new measures development and clinical research could be carried out in nursing intervention,in order to carry out more effec-tively personalized nursing for patients with cervical cancer.
