检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2014年
z1期
38-40
,共3页
李欣荣%刘岱璿%周小容%李慧源%唐春燕
李訢榮%劉岱璿%週小容%李慧源%唐春燕
리흔영%류대선%주소용%리혜원%당춘연
人乳头状瘤病毒%基因型%流式荧光杂交法%宫颈癌
人乳頭狀瘤病毒%基因型%流式熒光雜交法%宮頸癌
인유두상류병독%기인형%류식형광잡교법%궁경암
human papillomavirus%genotyping%flow-fluorescence hybridization%cervical cancer
目的：了解湖南省3451例宫颈人乳头状瘤病毒（HPV）感染状况和基因亚型分布。方法采用流式荧光杂交技术对湖南地区3451例女性进行 HPV 分型检测，并对 HPV DNA 亚型、感染率和年龄分布进行分析。结果3451例女性中检出 HPV 阳性917例，阳性率为26．57％。阳性感染者中，单一型感染最多，为665例，占72．52％，其中高危单一型感染者539例，占58．78％，低危单一型感染者126例，占13．74％，混合型感染者252例，占27．48％，主要为双重感染，占19．74％。19个与宫颈癌密切相关的 HPV 高危亚型阳性率为22．83％，7个低危亚型 HPV 阳性率为6．69％。感染排前8位的亚型分别是 HPV52（23．66％）、HPV16（16．58％）、HPV6（11．34％）、HPV58（11．01％）、HPV39（7．96％）、HPV33（6．43％）、HPV68（6．22％）、HPV18（5．78％）。在各年龄组中，＜25岁组感染率最高，为34．1％，＜25岁组与25～44岁组感染率差异有统计学意义（P ＜0．05）；45～54岁组感染率与25～44岁组差异有统计学意义（P ＜0．05）。结论湖南地区女性 HPV 感染以高危型感染为主，HPV52、16、6、58亚型是最主要的感染亚型，进行 HPV DNA 检测和分型对宫颈病变的防治具有重要意义。
目的：瞭解湖南省3451例宮頸人乳頭狀瘤病毒（HPV）感染狀況和基因亞型分佈。方法採用流式熒光雜交技術對湖南地區3451例女性進行 HPV 分型檢測，併對 HPV DNA 亞型、感染率和年齡分佈進行分析。結果3451例女性中檢齣 HPV 暘性917例，暘性率為26．57％。暘性感染者中，單一型感染最多，為665例，佔72．52％，其中高危單一型感染者539例，佔58．78％，低危單一型感染者126例，佔13．74％，混閤型感染者252例，佔27．48％，主要為雙重感染，佔19．74％。19箇與宮頸癌密切相關的 HPV 高危亞型暘性率為22．83％，7箇低危亞型 HPV 暘性率為6．69％。感染排前8位的亞型分彆是 HPV52（23．66％）、HPV16（16．58％）、HPV6（11．34％）、HPV58（11．01％）、HPV39（7．96％）、HPV33（6．43％）、HPV68（6．22％）、HPV18（5．78％）。在各年齡組中，＜25歲組感染率最高，為34．1％，＜25歲組與25～44歲組感染率差異有統計學意義（P ＜0．05）；45～54歲組感染率與25～44歲組差異有統計學意義（P ＜0．05）。結論湖南地區女性 HPV 感染以高危型感染為主，HPV52、16、6、58亞型是最主要的感染亞型，進行 HPV DNA 檢測和分型對宮頸病變的防治具有重要意義。
목적：료해호남성3451례궁경인유두상류병독（HPV）감염상황화기인아형분포。방법채용류식형광잡교기술대호남지구3451례녀성진행 HPV 분형검측，병대 HPV DNA 아형、감염솔화년령분포진행분석。결과3451례녀성중검출 HPV 양성917례，양성솔위26．57％。양성감염자중，단일형감염최다，위665례，점72．52％，기중고위단일형감염자539례，점58．78％，저위단일형감염자126례，점13．74％，혼합형감염자252례，점27．48％，주요위쌍중감염，점19．74％。19개여궁경암밀절상관적 HPV 고위아형양성솔위22．83％，7개저위아형 HPV 양성솔위6．69％。감염배전8위적아형분별시 HPV52（23．66％）、HPV16（16．58％）、HPV6（11．34％）、HPV58（11．01％）、HPV39（7．96％）、HPV33（6．43％）、HPV68（6．22％）、HPV18（5．78％）。재각년령조중，＜25세조감염솔최고，위34．1％，＜25세조여25～44세조감염솔차이유통계학의의（P ＜0．05）；45～54세조감염솔여25～44세조차이유통계학의의（P ＜0．05）。결론호남지구녀성 HPV 감염이고위형감염위주，HPV52、16、6、58아형시최주요적감염아형，진행 HPV DNA 검측화분형대궁경병변적방치구유중요의의。
Objective The aims of this study were to know the infection status and genotype distribution about the cervical human papillomavirus(HPV)of 3 451 cases in Hunan Province.Methods A total of 3 451 women infec-ted with the human papillomavirus in Hunan province were detected by the flow-fluorescence hybridization.The gen-otype,infection rate and age distribution were analyzed.Results Nine hundred and seventeen cases in 3 451 women had the positive results with HPV,and the positive rate was 26.57%.In the 917 cases with HPV,the main type was single type infection with the number as 665,and the percentage was 72.52%.Five hundred and thirty-nine of the 665 cases with single HPV genotype infection were infected with high-risk types,and the percentage was 58.78%. One hundred and twenty-six cases were infected with low-risk types,and the percentage was 13.74%.Two hundred and fifty-two cases were infected with mixed types,and the main infection type was dual type infection.The percent-age of mixed type infection was 19.74%.The total positive rate of 19 cervical cancer high-risk types was 22.83%, and the total positive rate of 7 cervical cancer low-risk types was 6.69%.The top eight HPV infection genotypes were HPV52 (23.66%),HPV16 (16.58%),HPV6 (11.34%),HPV58 (11.01%),HPV39 (7.96%),HPV33 (6. 43%),HPV68(6.22%),and HPV18(5.78%).Among the different age groups,the less than 25 years group had the highest infection rate with the percentage as 34.1%.The infection rates had significant difference between the group less than 25 years and the group from 25 years to 44 years.The infection rates had significant difference between the group from 45 years to 54 years and the group from 25 years to 44 years.Conclusion The main HPV infection types in Hunan province are high-risk type.The main HPV types are HPV52,16,6,58.The detection and genotyping of HPV DNA are important for the prevention of cervical cancer.
