CAJ | 학술논문

目的：比较滑膜炎颗粒在昆明小鼠和BALB/c小鼠中的镇痛作用。方法80只昆明小鼠、80只BALB/c小鼠各分为对照组、布洛芬组（0.078 g/kg）、滑膜炎颗粒大剂量组（13.98 g/kg）、滑膜炎颗粒中剂量组（6.99 g/kg）、滑膜炎颗粒小剂量组（3.50 g/kg），每组16只，雌雄各半。连续灌胃给药5 d，1次/d。第4天给药后1 h应用鼠尾光照仪，观察鼠尾受热后出现甩尾反应的潜伏期。末次给药后1 h腹腔注射醋酸制备疼痛模型，观察小鼠扭体潜伏期及扭体次数。结果滑膜炎颗粒各剂量组对昆明小鼠痛阈潜伏期、扭体次数无明显影响，滑膜炎颗粒中剂量组可减少雄性BALB/c小鼠痛阈潜伏期（3.93±0.76）min，与对照组的（4.84±1.16）min 比较，差异有统计学意义（P＜0.05）。滑膜炎颗粒小、中、大剂量组均能减少BALB/c小鼠扭体次数[分别为（8.56±6.19）次、（5.73±3.17）次、（6.88±4.59）次]，与对照组的（19.06±6.34）次比较，差异有统计学意义（P＜0.01）。结论滑膜炎颗粒各剂量组对化学刺激所致疼痛呈较好镇痛作用，镇痛作用无性别差异；昆明小鼠个体差异较大，不适宜镇痛药的药效学评价，应选用个体差异小的BALB/c小鼠制备疼痛动物模型。
목적：비교활막염과립재곤명소서화BALB/c소서중적진통작용。방법80지곤명소서、80지BALB/c소서각분위대조조、포락분조（0.078 g/kg）、활막염과립대제량조（13.98 g/kg）、활막염과립중제량조（6.99 g/kg）、활막염과립소제량조（3.50 g/kg），매조16지，자웅각반。련속관위급약5 d，1차/d。제4천급약후1 h응용서미광조의，관찰서미수열후출현솔미반응적잠복기。말차급약후1 h복강주사작산제비동통모형，관찰소서뉴체잠복기급뉴체차수。결과활막염과립각제량조대곤명소서통역잠복기、뉴체차수무명현영향，활막염과립중제량조가감소웅성BALB/c소서통역잠복기（3.93±0.76）min，여대조조적（4.84±1.16）min 비교，차이유통계학의의（P＜0.05）。활막염과립소、중、대제량조균능감소BALB/c소서뉴체차수[분별위（8.56±6.19）차、（5.73±3.17）차、（6.88±4.59）차]，여대조조적（19.06±6.34）차비교，차이유통계학의의（P＜0.01）。결론활막염과립각제량조대화학자격소치동통정교호진통작용，진통작용무성별차이；곤명소서개체차이교대，불괄의진통약적약효학평개，응선용개체차이소적BALB/c소서제비동통동물모형。
Objective To compare the analgesic effect ofHuamoyan Keli(HMYKL) between Kunming mice and BALB/c mice.Methods Eighty Kunming mice and eighty BALB/c mice were randomly divided into five groups, respectively: a control group, a ibuprofen group, a HMYKL high-dose group(13.98 g crude dru g/kg), HMYKL middle-dose group(6.99 g crude drug/kg)and a HMYKL low-dose group(3.50 g crude dru g/kg). There were 16 mice in each group with 8 male mice and 8 female mice. Drugs were administered intragastrically daily for 5 days. After 1 h of drug treatment on day 4, the latency of tail-flick response was evaluated using illuminated pain measurement instrument. After the last drug treatment, pain model was established by i.p. acetic acid, writhing latency and writhing times were recorded to evaluate the analgesic effect of HMYKL.Results In tail-flick test, there was no statistical difference among male and female Kunming mice in the HMYKL groups. Among male BALB/c mice, the latency in HMYKL middle-dose group was significantly longer than that in the control group(4.84±1.16 minvs. 3.93±0.76 min,P<0.05). In writhing test, compared with control group(19.06±6.34), the writhing times among BALB/c mice were decreased in HMYKL high-dose group(8.56±6.19), HMYKL middle-dose group(5.73±3.17), HMYKL low-dose group(6.88±4.59)(allP<0.01).Conclusion All dose groups of HMYKL showed good analgesic effect on the pain induced by chemical stimulation and there was no sex difference. Kunming mice were not suitable for the evaluation of the analgesic pharmacodynamics because of their large individual difference. On contrast, BALB/c mice which had less individual difference could be used to produce the model of pain.