国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2014年
11期
1006-1008
,共3页
李建民%杨袆楠%张宁%王业秋%李铁男%王发善%刘海洋
李建民%楊袆楠%張寧%王業鞦%李鐵男%王髮善%劉海洋
리건민%양위남%장저%왕업추%리철남%왕발선%류해양
逍遥散%药物性肝损伤%四氯化碳%防护药(中药)
逍遙散%藥物性肝損傷%四氯化碳%防護藥(中藥)
소요산%약물성간손상%사록화탄%방호약(중약)
Xiaoyaosan%Drug-induced liver injury%Carbon tetrachloride%Protective agents(TCD)
目的:观察逍遥散对四氯化碳(CCl4)致小鼠急性肝损伤的肝脏保护作用。方法将30只小鼠按照随机数字表法分为空白对照组、模型组、逍遥散组,逍遥散组灌胃逍遥散,其余2组灌胃等量蒸馏水。给药后7 d,腹腔注射CCl4花生油溶液诱导小鼠急性肝损伤,16 h后取材,检测小鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)浓度,并取肝组织做病理学观察。结果模型组小鼠 ALT、AST、MDA 水平明显升高,SOD 水平明显降低[分别为(136.46±15.75)U/L、(145.37±16.39)U/L、(17.48±3.45)nmol/mg、(261.60±20.29)U/mg],与空白对照组比较[分别为(22.96±6.23)U/L、(31.89±7.26)U/L、(4.22±1.08) nmol /mg、(336.73±25.34)U/mg],差异有统计学意义(P<0.01)。逍遥散组ALT、AST、MDA水平明显降低,SOD水平升高[分别为(89.38±6.96)U/L、(119.04±20.44)U/L、(10.30±2.22)nmol/mg、(304.77±31.71)U/mg],与模型组比较差异有统计学意义(P<0.01或0.05)。结论逍遥散对CCl4致小鼠急性肝损伤有保护作用。
目的:觀察逍遙散對四氯化碳(CCl4)緻小鼠急性肝損傷的肝髒保護作用。方法將30隻小鼠按照隨機數字錶法分為空白對照組、模型組、逍遙散組,逍遙散組灌胃逍遙散,其餘2組灌胃等量蒸餾水。給藥後7 d,腹腔註射CCl4花生油溶液誘導小鼠急性肝損傷,16 h後取材,檢測小鼠血清丙氨痠轉氨酶(ALT)、天鼕氨痠轉氨酶(AST)、肝組織丙二醛(MDA)、超氧化物歧化酶(SOD)濃度,併取肝組織做病理學觀察。結果模型組小鼠 ALT、AST、MDA 水平明顯升高,SOD 水平明顯降低[分彆為(136.46±15.75)U/L、(145.37±16.39)U/L、(17.48±3.45)nmol/mg、(261.60±20.29)U/mg],與空白對照組比較[分彆為(22.96±6.23)U/L、(31.89±7.26)U/L、(4.22±1.08) nmol /mg、(336.73±25.34)U/mg],差異有統計學意義(P<0.01)。逍遙散組ALT、AST、MDA水平明顯降低,SOD水平升高[分彆為(89.38±6.96)U/L、(119.04±20.44)U/L、(10.30±2.22)nmol/mg、(304.77±31.71)U/mg],與模型組比較差異有統計學意義(P<0.01或0.05)。結論逍遙散對CCl4緻小鼠急性肝損傷有保護作用。
목적:관찰소요산대사록화탄(CCl4)치소서급성간손상적간장보호작용。방법장30지소서안조수궤수자표법분위공백대조조、모형조、소요산조,소요산조관위소요산,기여2조관위등량증류수。급약후7 d,복강주사CCl4화생유용액유도소서급성간손상,16 h후취재,검측소서혈청병안산전안매(ALT)、천동안산전안매(AST)、간조직병이철(MDA)、초양화물기화매(SOD)농도,병취간조직주병이학관찰。결과모형조소서 ALT、AST、MDA 수평명현승고,SOD 수평명현강저[분별위(136.46±15.75)U/L、(145.37±16.39)U/L、(17.48±3.45)nmol/mg、(261.60±20.29)U/mg],여공백대조조비교[분별위(22.96±6.23)U/L、(31.89±7.26)U/L、(4.22±1.08) nmol /mg、(336.73±25.34)U/mg],차이유통계학의의(P<0.01)。소요산조ALT、AST、MDA수평명현강저,SOD수평승고[분별위(89.38±6.96)U/L、(119.04±20.44)U/L、(10.30±2.22)nmol/mg、(304.77±31.71)U/mg],여모형조비교차이유통계학의의(P<0.01혹0.05)。결론소요산대CCl4치소서급성간손상유보호작용。
Objective To investigate the liver protective effects of Xiaoyaosan in carbon tetrachloride-induced acute liver injury in mice.Methods Thirty mice were randomly divided into a control group, a model group, and aXiaoyaosan group, with 10 mice in each group. The mice in theXiaoyaosan group were intragastrically administrated withXiaoyaosan, the mice in the remaining two groups were fed with an equal volume of distilled water. After 7 days, acute liver injury were inducedvia intraperitoneal injection of carbon tetrachloride peanut oil solution. The serum levels of alanine transaminase(ALT), aspartate transaminase (AST), malondialdehyde(MDA)and superoxide dismutase(SOD)were measured, and pathological changes of liver tissue was tested after 16 hours.Results The serum levels of ALT(136.46±15.75 U/Lvs. 22.96±6.23 U/L), AST(145.37±16.39 U/Lvs. 31.89±7.26 U/L), and MDA level in the liver tissue(17.48±3.45 nmol/mgvs. 4.22±1.08 nmol/mg)in the model group were significantly higher than those in the control group(allP<0.01), SOD level in the liver tissue significantly lower than that in the control group(261.60±20.29 U/mgvs. 336.73±25.34 U/mg,P<0.01). The serum levels of ALT(89.38±6.96 U/L,P<0.01), AST(119.04±20.44 U/L, P<0.05), MDA level(10.30±2.22 nmol/mg,P<0.01) in the liver tissue in theXiaoyaosan group levels were significantly lower than those in the model group, and SOD level(304.77±31.71 U/mg,P<0.01) in the liver tissue were significantly higher than that in the model group.ConclusionXiaoyaosan has liver protective effects in carbon tetrachloride-induced acute liver injury in mice.