国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2014年
11期
1002-1005
,共4页
潘金%毕洪钟%王伟%琚坚
潘金%畢洪鐘%王偉%琚堅
반금%필홍종%왕위%거견
阴行草%肝纤维化%药物性肝损伤%四氯化碳%肿瘤坏死因子-α%防护药(中药)
陰行草%肝纖維化%藥物性肝損傷%四氯化碳%腫瘤壞死因子-α%防護藥(中藥)
음행초%간섬유화%약물성간손상%사록화탄%종류배사인자-α%방호약(중약)
Siphonostegia chinensis%Liver Fibrosis%Drug-Induced Liver Injury%Carbon Tetrachloride%Tumor Necrosis Factor-α%Protective Agents(TCD)
目的：探讨阴行草水煎液对四氯化碳（CCl4）诱导的肝纤维化大鼠的肝脏保护作用。方法120只雄性SD大鼠随机分为空白对照组、肝纤维化模型组、阴行草组、丹参组、虎杖组及易善复组，每组20只。除空白对照组外，其余5组以CCl4皮下注射诱导肝纤维化；除空白对照组、肝纤维化模型组外，其余4组大鼠分别给予阴行草、丹参、虎杖及易善复10 ml/kg灌胃，1次/d。10周后，采用酶联免疫吸附法检测血清肿瘤坏死因子-α（TNF-α）水平；光镜下观察肝脏病理组织学改变，并进行肝纤维化分期。结果与肝纤维化模型组比较，阴行草组可降低肝纤维化程度（P＜0.01），且阴行草组、虎杖组、丹参组和易善复组之间肝纤维化组织学分期无显著差异（P＞0.05）。与肝纤维化模型组 TNF-α浓度（368.06±24.90）U/L比较，阴行草组（336.61±20.20）U/L明显降低（P＜0.01）；阴行草组与丹参组（337.81±21.04）U/L、虎杖组（338.95±24.43）U/L、易善复组（337.11±23.64）U/L比较差异均无统计学意义（P＞0.05）。结论阴行草水煎液对CCl4诱导的肝纤维化大鼠肝脏有保护作用。
目的：探討陰行草水煎液對四氯化碳（CCl4）誘導的肝纖維化大鼠的肝髒保護作用。方法120隻雄性SD大鼠隨機分為空白對照組、肝纖維化模型組、陰行草組、丹參組、虎杖組及易善複組，每組20隻。除空白對照組外，其餘5組以CCl4皮下註射誘導肝纖維化；除空白對照組、肝纖維化模型組外，其餘4組大鼠分彆給予陰行草、丹參、虎杖及易善複10 ml/kg灌胃，1次/d。10週後，採用酶聯免疫吸附法檢測血清腫瘤壞死因子-α（TNF-α）水平；光鏡下觀察肝髒病理組織學改變，併進行肝纖維化分期。結果與肝纖維化模型組比較，陰行草組可降低肝纖維化程度（P＜0.01），且陰行草組、虎杖組、丹參組和易善複組之間肝纖維化組織學分期無顯著差異（P＞0.05）。與肝纖維化模型組 TNF-α濃度（368.06±24.90）U/L比較，陰行草組（336.61±20.20）U/L明顯降低（P＜0.01）；陰行草組與丹參組（337.81±21.04）U/L、虎杖組（338.95±24.43）U/L、易善複組（337.11±23.64）U/L比較差異均無統計學意義（P＞0.05）。結論陰行草水煎液對CCl4誘導的肝纖維化大鼠肝髒有保護作用。
목적：탐토음행초수전액대사록화탄（CCl4）유도적간섬유화대서적간장보호작용。방법120지웅성SD대서수궤분위공백대조조、간섬유화모형조、음행초조、단삼조、호장조급역선복조，매조20지。제공백대조조외，기여5조이CCl4피하주사유도간섬유화；제공백대조조、간섬유화모형조외，기여4조대서분별급여음행초、단삼、호장급역선복10 ml/kg관위，1차/d。10주후，채용매련면역흡부법검측혈청종류배사인자-α（TNF-α）수평；광경하관찰간장병리조직학개변，병진행간섬유화분기。결과여간섬유화모형조비교，음행초조가강저간섬유화정도（P＜0.01），차음행초조、호장조、단삼조화역선복조지간간섬유화조직학분기무현저차이（P＞0.05）。여간섬유화모형조 TNF-α농도（368.06±24.90）U/L비교，음행초조（336.61±20.20）U/L명현강저（P＜0.01）；음행초조여단삼조（337.81±21.04）U/L、호장조（338.95±24.43）U/L、역선복조（337.11±23.64）U/L비교차이균무통계학의의（P＞0.05）。결론음행초수전액대CCl4유도적간섬유화대서간장유보호작용。
Objective To investigate the liver protective effects of Siphonostegia chinensis decoction in rats with liver fibrosis induced by carbon tetrachloride(CCl4).Methods A total of 120 male SD rats were randomly divided into six groups: a blank control group, a model group, a Siphonostegia chinensis decoction group, a Salvia miltiorrhiza decoction group, a Rhizoma polygoni cuspidati decoction group and a polyene phosphatidylcholine capsules group, with 20 rats in each group. The model of liver fibrosis in rats was induced by subcutaneous injection with CCl4, except the blank group. Except rats in the blank control group and the model group, the rats in the other four groups were treated with Siphonostegia chinensis decoction, Salvia miltiorrhiza decoction, Rhizoma polygoni cuspidati decoction and polyene phosphatidylcholine capsules(10 ml/kg). The serum level of tumor necrosis factor-α(TNF-α)was determined with enzyme-linked immunosorbent assay, the fibrosis stage assessment were determined by histopathological examination.Results Compared with the model group, Siphonostegia chinensis decoction group significantly reduced the stage of liver fibrosis(P<0.01)and there were no obvious difference in liver fibrosis stage among the Salvia miltiorrhiza decoction group, Rhizoma polygoni cuspidati decoction group and polyene phosphatidylcholine capsules group, and the Siphonostegia chinensis decoction group(allP>0.05).Compared with liver fibrosis model group(368.06±24.90)U/L, the serum level of TNF-α in the Siphonostegia chinensis decoction group(336.61± 20.20)U/L significantly reduced(P<0.01), and there were no obvious difference among the Siphonostegia chinensis decoction group, the Salvia miltiorrhiza decoction group(337.81±21.04)U/L, the Rhizoma polygoni cuspidati decoction group(338.95±24.43)U/L and the polyene phosphatidylcholine capsules group(337.11± 23.64) U/L(allP>0.05).Conclusion Siphonostegia chinensis decoction has certain liver protective effect in rats with liver fibrosis induced by CCl4.
