CAJ | 학술논문

目的：探讨 miRNA-143对前列腺癌细胞增殖的影响及其作用机制。方法通过RT-PCR和Western blot方法检测miRNA-143及其靶基因KRAS在前列腺癌组织中的表达；用MTT方法和集落形成实验研究 miRNA-143对激素非依赖性前列腺癌 DU145细胞增殖的影响。结果 miRNA-143及其靶基因 KRAS 在前列腺癌组织中的表达呈负相关；miRNA-143高表达对DU145细胞的增殖具有抑制作用；DU145细胞中miRNA-143高表达抑制KRAS-ERK信号通路的激活及Cyclin D1的表达。结论 miRNA-143通过RAS/MAPK信号通路抑制前列腺癌的增殖，为激素非依赖性前列腺癌治疗提供了分子生物学基础。
목적：탐토 miRNA-143대전렬선암세포증식적영향급기작용궤제。방법통과RT-PCR화Western blot방법검측miRNA-143급기파기인KRAS재전렬선암조직중적표체；용MTT방법화집락형성실험연구 miRNA-143대격소비의뢰성전렬선암 DU145세포증식적영향。결과 miRNA-143급기파기인 KRAS 재전렬선암조직중적표체정부상관；miRNA-143고표체대DU145세포적증식구유억제작용；DU145세포중miRNA-143고표체억제KRAS-ERK신호통로적격활급Cyclin D1적표체。결론 miRNA-143통과RAS/MAPK신호통로억제전렬선암적증식，위격소비의뢰성전렬선암치료제공료분자생물학기출。
Objective To verify its effect and mechanisms on prostate cancer cell proliferation. Methods miRNA-143 and its target gene KRAS expression was measured by real-time PCR and western blotting and effects of miRNA-143 in cell proliferation and migration were evaluated by MTT and colony formation. Results An inverse correlation between miRNA-143 and KRAS expression in prostate cancer tissues. Over-expression of miRNA-143 in DU145 cells suppressed their proliferation by targeting RAS/MAPK pathway. Conclusion miRNA-143 plays an important role in prostate cancer cell proliferation by suppressing KRAS and subsequent inactivation of MAPK pathway, which provide a new approach for HRPC treatment.