CAJ | 학술논문

目的：研究幽门螺杆菌(Helicobacterpylori,HP)感染中胃黏膜白细胞介素mRNA水平。方法选取2010年10月～2013年6月在中山市民众医院接受检查治疗的慢性胃炎患者52例，将收治的HP阳性患者分别采用real-time PCR、PCR-荧光探针法及酶联免疫吸附实验夹心法检测患者胃黏膜白细胞介素mRNA(IL-6、IL-1β)的水平，记录治疗前后mRNA(IL-6、IL-1β)水平，探讨与幽门螺杆菌感染相关性。结果患者胃黏膜白细胞介素mRNA水平随着幽门螺旋杆菌感染加重而升高，尤其是IL-6水平，随着感染程度的不断恶化。同时，治疗前后相比患者的感染程度从降低，其血清与胃黏膜蛋白中的IL-6活性水平降低，而IL-1β水平无明显变化。结论幽门螺杆菌感染对mRNA的影响不显著，而IL-6与幽门螺杆菌的感染有着密切的关系。
목적：연구유문라간균(Helicobacterpylori,HP)감염중위점막백세포개소mRNA수평。방법선취2010년10월～2013년6월재중산시민음의원접수검사치료적만성위염환자52례，장수치적HP양성환자분별채용real-time PCR、PCR-형광탐침법급매련면역흡부실험협심법검측환자위점막백세포개소mRNA(IL-6、IL-1β)적수평，기록치료전후mRNA(IL-6、IL-1β)수평，탐토여유문라간균감염상관성。결과환자위점막백세포개소mRNA수평수착유문라선간균감염가중이승고，우기시IL-6수평，수착감염정도적불단악화。동시，치료전후상비환자적감염정도종강저，기혈청여위점막단백중적IL-6활성수평강저，이IL-1β수평무명현변화。결론유문라간균감염대mRNA적영향불현저，이IL-6여유문라간균적감염유착밀절적관계。
Objective Helicobacter pylori (HP) infection in gastric interleukin mRNA levels.Methods A retrospective analysis of our hospital for examination and treatment of 52 cases of chronic gastriti from October 2010 to June 2013,HP-positive patients were treated using real-time PCR, PCR-lfuorescence probe method and sandwich enzyme-linked immunosorbent assay was used to detect patients with gastric interleukin m RNA (IL-6, IL-1β) levels,recorded before and after treatment m RNA (IL-6, IL-1β) levels. To investigate the correlation between Helicobacter pylori infection. Results The study showed that patients with gastric interleukin m RNA levels increased with H. pylori infection and elevated levels of IL-6 in particular, with the extent of the infection worsening. Meanwhile, the degree of infection as compared to the patients before and after treatment from a lower decrease in the serum of gastric mucosal protein levels of IL-6 activity. The IL-1β levels were not signiifcantly changed.Conclusion The effect on Helicobacter pylori infection m RNA is not signiifcant,while IL-6 and Helicobacter pylori infection is closely related.