CAJ | 학술논문

利用共沉淀法合成了磁性Fe3 O4纳米粒子,进一步表面功能化,合成Fe3 O4@Au磁性纳米粒子提高粒子表面的亲和性。在高亲和力的金壳表面自组装L-半胱氨酸-GA3,将其滴涂在磁控玻碳电极表面,电聚合L-半胱氨酸制得对GA3具有特异性识别能力的MIP/Fe3 O4@Au修饰电极。对Fe3 O4@Au磁性纳米粒子的表面形态及粒度分布进行了透射电镜分析,对GA3, MIP及nMIP的结构及成分进行了红外光谱对比分析。利用电化学方法对测试体系的工作条件进行了优化。研究表明,当电聚合圈数为30圈,以乙酸-甲醇(1：8, V/V)作为洗脱液、洗脱时间为5 min、重吸附时间为7 min时,传感器具有较高的稳定性,且对GA3具有较好的识别效果。结果表明,探针离子K3[Fe(CN)6]/K4[Fe(CN)6]的氧化峰电流值与GA3浓度在1.0×10-11~1.0×10-8 mol/L范围内呈线性关系,检出限为2.6×10-12 mol/L。此传感器已应用于啤酒中GA3的检测。
이용공침정법합성료자성Fe3 O4납미입자,진일보표면공능화,합성Fe3 O4@Au자성납미입자제고입자표면적친화성。재고친화력적금각표면자조장L-반광안산-GA3,장기적도재자공파탄전겁표면,전취합L-반광안산제득대GA3구유특이성식별능력적MIP/Fe3 O4@Au수식전겁。대Fe3 O4@Au자성납미입자적표면형태급립도분포진행료투사전경분석,대GA3, MIP급nMIP적결구급성분진행료홍외광보대비분석。이용전화학방법대측시체계적공작조건진행료우화。연구표명,당전취합권수위30권,이을산-갑순(1：8, V/V)작위세탈액、세탈시간위5 min、중흡부시간위7 min시,전감기구유교고적은정성,차대GA3구유교호적식별효과。결과표명,탐침리자K3[Fe(CN)6]/K4[Fe(CN)6]적양화봉전류치여GA3농도재1.0×10-11~1.0×10-8 mol/L범위내정선성관계,검출한위2.6×10-12 mol/L。차전감기이응용우비주중GA3적검측。
ThemagneticFe3O4nanoparticlesweresynthesizedbyco-precipitationmethod,andthenmagnetic Fe3 O4@Au nanoparticle was synthesized to improve the affinity of particle surface. L-Cys-GA3 was grafted on the surface of gold clad by self-assembly method, and then dropped it on glassy carbon electrode, for further manufacture of MIP/Fe3 O4@Au by using electropolymerzation L-Cys. The surface morphology and particle size distribution of Fe3 O4@Au were studied by TEM. The structure and composition of gibberellins A3, MIP and nMIP were studied by IR. The test system was optimized, and the results showed that when the cycles of electropolymerization was 30, acetic acid:methanol (1:8, V/V) was chosen as eluent, elution time was optimized for 5 min and rebinding time for 7 min, the sensor got a high stability and good recognition ability for gibberellins A3 . The concentration of gibberellins A3 in the range of 1 . 0 × 10-11-1 . 0 × 10-8 mol/L had a relationship with the oxidation peak current of probe, with the detection limit of 2. 57×10-12 mol/L. The sensor was successfully used for the determination of GA3 in beer sample.