CAJ | 학술논문

在水温26±0.5℃下，研究以20~100mg/L之间5个浓度梯度的MS-222（tricaine methane sulfonate）对体质量255.87±30.31g野生长薄鳅Leptobotia elongate的麻醉效果。结果表明：在20mg/L的MS-222下，长薄鳅的活动量明显下降，保持镇静，维持身体平衡和正常体位，麻醉3h后，在清水中几分钟内便可恢复正常游动状态，适合于长途运输；40~80mg/L的MS-222对长薄鳅的麻醉作用较强，适合于短时间实验操作，如外科手术、体外标记、形态学测量等；当MS-222的浓度达到100mg/L时，短时间内长薄鳅便进入深度麻醉甚至休克状态，若不及时进行复苏，鱼易死亡。实验证明，用MS-222麻醉长薄鳅较快、复苏时间短、安全性能高，是较理想的麻醉剂。
재수온26±0.5℃하，연구이20~100mg/L지간5개농도제도적MS-222（tricaine methane sulfonate）대체질량255.87±30.31g야생장박추Leptobotia elongate적마취효과。결과표명：재20mg/L적MS-222하，장박추적활동량명현하강，보지진정，유지신체평형화정상체위，마취3h후，재청수중궤분종내편가회복정상유동상태，괄합우장도운수；40~80mg/L적MS-222대장박추적마취작용교강，괄합우단시간실험조작，여외과수술、체외표기、형태학측량등；당MS-222적농도체도100mg/L시，단시간내장박추편진입심도마취심지휴극상태，약불급시진행복소，어역사망。실험증명，용MS-222마취장박추교쾌、복소시간단、안전성능고，시교이상적마취제。
The anesthetic effects of MS-222, tricaine methane sulfonate, on wild elongate loach Leptobotia elongate with body weight of(255.87±30.31) g were investigated at a dose of 20~100 mg/L at water temperature of 26±0.5℃. The results showed that the fish had moderate effects for long distance transportation at 20 mg/L of MS-222, with slowing movement, calmness and equilibrium body, and being recovery in fresh water 3 h after anesthesia. The strong anesthetic effects on the fish were observed at 40~80 mg/L of MS-222 at which is appropriate for quick handling such as surgical operation, external marking, and morphometric measurement. The fish became deep anesthesia, even shock state, in a short period of time, unable recovery in time, or death at a dose of over 80 mg/L for MS-222. The findings indicate that MS-222 is feasible for anesthesia of elongate loach which can be induced anesthesia and recovered rapidly within a wide safety concentration range of MS-222.