水产学杂志
水產學雜誌
수산학잡지
CHINESE JOURNAL OF FISHERIES
2014年
5期
24-27
,共4页
谷伟%张永泉%户国%徐革锋%王炳谦
穀偉%張永泉%戶國%徐革鋒%王炳謙
곡위%장영천%호국%서혁봉%왕병겸
虹鳟%精子%超微结构
虹鱒%精子%超微結構
홍준%정자%초미결구
rainbow trout%spermatozoon%ultrastructure
应用扫描和透射电镜观察虹鳟Oncorhynchus mykiss精子的形态和超微结构。虹鳟精子由头部和尾部组成。头部呈椭球形,主要结构是细胞核,核前端无顶体,后端有较深的植入窝,凹入细胞核的1/3,核中染色质致密,有不规则的网络状间隙,植入窝中有袖套和中心粒复合体。中心粒复合体主要由近端中心粒和远端中心粒构成,二者垂直分布,前者为典型的9组三联微管结构,后者向下延伸形成轴丝。袖套与细胞核后端相连,含有丰富的线粒体和囊泡;精子尾部细长,主要结构是轴丝,为典型的“9+2”微管结构,轴丝为许多囊泡包围,质膜向外突出两个侧鳍,侧鳍上下对称,但大小不同。
應用掃描和透射電鏡觀察虹鱒Oncorhynchus mykiss精子的形態和超微結構。虹鱒精子由頭部和尾部組成。頭部呈橢毬形,主要結構是細胞覈,覈前耑無頂體,後耑有較深的植入窩,凹入細胞覈的1/3,覈中染色質緻密,有不規則的網絡狀間隙,植入窩中有袖套和中心粒複閤體。中心粒複閤體主要由近耑中心粒和遠耑中心粒構成,二者垂直分佈,前者為典型的9組三聯微管結構,後者嚮下延伸形成軸絲。袖套與細胞覈後耑相連,含有豐富的線粒體和囊泡;精子尾部細長,主要結構是軸絲,為典型的“9+2”微管結構,軸絲為許多囊泡包圍,質膜嚮外突齣兩箇側鰭,側鰭上下對稱,但大小不同。
응용소묘화투사전경관찰홍준Oncorhynchus mykiss정자적형태화초미결구。홍준정자유두부화미부조성。두부정타구형,주요결구시세포핵,핵전단무정체,후단유교심적식입와,요입세포핵적1/3,핵중염색질치밀,유불규칙적망락상간극,식입와중유수투화중심립복합체。중심립복합체주요유근단중심립화원단중심립구성,이자수직분포,전자위전형적9조삼련미관결구,후자향하연신형성축사。수투여세포핵후단상련,함유봉부적선립체화낭포;정자미부세장,주요결구시축사,위전형적“9+2”미관결구,축사위허다낭포포위,질막향외돌출량개측기,측기상하대칭,단대소불동。
The ultrastructure of spermatozoon was observed in rainbow trout Oncorhynchus mykiss under scanning and transmission electron microscopes. The spermatozoa were found to consist of two parts, a globe-like head containing nucleus and tail. There was no acrosome at the anterior end of the nucleus, whereas there was an implantation fossa, which was deep and inset the nucleus 1/3, at the posterior end of the nucleus filled with electron dense chromatin materials and contained irregularly netlike gap. The implantation fossa had centriolar complex consisted of proximal centriole, which was made up of nine triplets of pripheral microtubules, distal centriole, which was arranged at a right angle to each other, and sleeve, which was connected to the postenor of the nuc1eus. There were some mitochondria and vesicles in the sleeve. The thin and long tail had undulating and symmetrically arranging lateral fins, primarily the axoneme with typical mode1 of“9+2”. There were many vesicles outside the axoneme.