CAJ | 학술논문

为选育抗疱疹病毒的德国镜鲤（Cyprinus carpio var. mirror carp）新品种，2012年在F1抗病家系基础上，构建27个家系，同池养殖。本研究采集经疱疹病毒感染存活个体，应用多态性微卫星标记对构建的27个德国镜鲤家系进行鉴定和遗传结构分析。结果显示：（1）4个位点累计排除率为99.9%，亲本与子代的配对率为100%（P=0.05）；（2）在27个家系的4个位点中检测到32个等位基因，每个位点7~10个等位基因，期望杂合度为0.6796~0.8019，平均期望杂合度为0.7510；（3）8个家系处于Hardy-Weinberg平衡状态下，19个家系处于不平衡状态下（P<0.05），其中有15个家系为极显著不平衡（P<0.01）；（4）27个家系间遗传距离为0.1751~0.8772，平均值为0.4425，聚类分析中，27个家系主要聚在两个分支上，其它家系分散于不同分支。结果表明：27个家系具有较高的遗传多样性，家系间遗传分化明显，大部分家系因病毒选择作用偏离Hardy-Weinberg平衡。家系群体遗传结构研究结果将为下一步品种选育工作提供重要的理论依据和技术支持。
위선육항포진병독적덕국경리（Cyprinus carpio var. mirror carp）신품충，2012년재F1항병가계기출상，구건27개가계，동지양식。본연구채집경포진병독감염존활개체，응용다태성미위성표기대구건적27개덕국경리가계진행감정화유전결구분석。결과현시：（1）4개위점루계배제솔위99.9%，친본여자대적배대솔위100%（P=0.05）；（2）재27개가계적4개위점중검측도32개등위기인，매개위점7~10개등위기인，기망잡합도위0.6796~0.8019，평균기망잡합도위0.7510；（3）8개가계처우Hardy-Weinberg평형상태하，19개가계처우불평형상태하（P<0.05），기중유15개가계위겁현저불평형（P<0.01）；（4）27개가계간유전거리위0.1751~0.8772，평균치위0.4425，취류분석중，27개가계주요취재량개분지상，기타가계분산우불동분지。결과표명：27개가계구유교고적유전다양성，가계간유전분화명현，대부분가계인병독선택작용편리Hardy-Weinberg평형。가계군체유전결구연구결과장위하일보품충선육공작제공중요적이론의거화기술지지。
In order to breed resistance to CyHV-3 new variety of mirror common carp (Cyprinus carpio var. mirror carp), 27 families were constructed in the F1 families and were cultured in the same ponds in 2012. In the study, the survivors were sampled and the ge-netic structure and identification of 27 families were inferred by microsatellite markers. The accumulative exclusion rate using four mi-crosatellite loci was 99.9%and the assignment rate between parents and offspring was 100%with confidence of 95%. Thirty-two alle-les were found in the families at a rate of 7~10 alleles per locus. The expected heterozygosis was ranged from 0.6796 to 0.8019 with average of 0.7510. Hardy-Weinberg equilibrium analysis showed that 19 families were in disequilibrium mode (P<0.05),in which 15 were significantly disequilibrium (P<0.01). The genetic distance among 27 families were from 0.1751 to 0.8772 with an average value of 0.4452. The family clustering showed that most families were converged into two branches in topology tree and others scattered in different branches, indicating that there is rich genetic diversity in the families. The genetic differentiation among families was found significant, in which most were deviated from Hardy-Weinberg equilibrium because of the virus selection. The findings will be impor-tant for the selective breeding to CyHV-3 in the future.