CAJ | 학술논문

采用聚丙烯酰胺凝胶电泳技术对高寒鲤Cyprinus carpio、荷包红鲤Cyprinus carpio wuyuanensis、松浦鲤Cyprinus carpio Songpu和德国镜鲤眼（E）、心（H）、肾（K）、肝胰脏（L），和肌肉（M）组织中超氧物歧化酶（SOD）、乙醇脱氢酶（ADH）、α-淀粉酶（α-AMY）、过氧化物酶（POD）、苹果酸酶（ME）、葡萄糖-6-磷酸脱氢酶（G-6-PDH）、乳酸脱氢酶（LDH）、酯酶（EST），和山梨醇脱氢酶（SDH）等9种同工酶进行了电泳分析。结果表明：4种鲤同工酶谱具有明显的组织特异性，其中肝胰脏和肾脏表达最为显著。4个鲤群体9种同工酶共记录了17个基因座位，多态座位比例P平均为27.865%，平均预期杂合度He’介于0.1329～0.1451之间。 Shannon’s多样性指数显示，4个群体的遗传变异由高到低依次为：荷包红鲤>高寒鲤>松浦鲤>德国镜鲤。4种鲤具有较丰富的遗传多样性，较高的遗传变异水平，对种质资源的研究开发及利用有重要意义。
채용취병희선알응효전영기술대고한리Cyprinus carpio、하포홍리Cyprinus carpio wuyuanensis、송포리Cyprinus carpio Songpu화덕국경리안（E）、심（H）、신（K）、간이장（L），화기육（M）조직중초양물기화매（SOD）、을순탈경매（ADH）、α-정분매（α-AMY）、과양화물매（POD）、평과산매（ME）、포도당-6-린산탈경매（G-6-PDH）、유산탈경매（LDH）、지매（EST），화산리순탈경매（SDH）등9충동공매진행료전영분석。결과표명：4충리동공매보구유명현적조직특이성，기중간이장화신장표체최위현저。4개리군체9충동공매공기록료17개기인좌위，다태좌위비례P평균위27.865%，평균예기잡합도He’개우0.1329～0.1451지간。 Shannon’s다양성지수현시，4개군체적유전변이유고도저의차위：하포홍리>고한리>송포리>덕국경리。4충리구유교봉부적유전다양성，교고적유전변이수평，대충질자원적연구개발급이용유중요의의。
Superoxide dismutase(SOD), alcohol dehydrogenase(ADH),α-Amylase(α-AMY), peroxidase(POD), malic enzyme (ME), glucose-6-phosphate dehydrogenase(G-6-PDH), lacrate dehydrogenase(LDH), esterase(EST), and succinate dehydrogenase (SDH) were investigated in 5 tissues(eye, muscle, hepatopancreas, heart and kidney) of Cold-tolerant common carp Cyprinus carpio, red carp Cyprinus carpio wuyuanensis, Songpu carp Cyprinus carpio Songpu and Germany mirror carp by vertical polyacrylamide gel-electrophoresis. The results showed that the 4 isoenzymes exhibited a tissue specific expression apparently,stronger expression in hep-atopancreas and kidney tissue. There were 17 gene loci in the 9 isozymes in the four carp populations, with polymorphic locus rate P=27.865%, expected heterozygosity He’ from 0.1329 to 0.1451. Shannon's Information index revealed that the order of the genetic vari-ation was ranged from high to low as red carp>cold-tolerant common carp>Songpu carp>Germany mirror carp. The rich genetic di-versity and higher genetic variation in these four carp play an important role in germplasm research and utilization.