花生学报
花生學報
화생학보
PEANUT SCIENCE AND TECHNOLOGY
2014年
3期
1-6
,共6页
葛姗姗%唐桂英%陈霞霞%付旭%柳展基%毕玉平
葛姍姍%唐桂英%陳霞霞%付旭%柳展基%畢玉平
갈산산%당계영%진하하%부욱%류전기%필옥평
花生%FT%功能预测
花生%FT%功能預測
화생%FT%공능예측
peanut%FT%functional prediction
FT (Flowering Locus T )作为“成花素”在成花转变及促进开花上起重要作用。本研究利用分子克隆和 RT-PCR 方法,从花生中克隆了一个与 FT 同源的 cDNA 序列,命名为 AhFT,该序列长度为893 bp,开放阅读框长度为534 bp,推测编码蛋白含有177个氨基酸,分子量为19.95 kD,等电点为6.9。通过与其他植物 PEBP 蛋白序列的比对,发现花生 FT 基因属于 FT-Like 家族,可能在控制植物开花中起重要作用。
FT (Flowering Locus T )作為“成花素”在成花轉變及促進開花上起重要作用。本研究利用分子剋隆和 RT-PCR 方法,從花生中剋隆瞭一箇與 FT 同源的 cDNA 序列,命名為 AhFT,該序列長度為893 bp,開放閱讀框長度為534 bp,推測編碼蛋白含有177箇氨基痠,分子量為19.95 kD,等電點為6.9。通過與其他植物 PEBP 蛋白序列的比對,髮現花生 FT 基因屬于 FT-Like 傢族,可能在控製植物開花中起重要作用。
FT (Flowering Locus T )작위“성화소”재성화전변급촉진개화상기중요작용。본연구이용분자극륭화 RT-PCR 방법,종화생중극륭료일개여 FT 동원적 cDNA 서렬,명명위 AhFT,해서렬장도위893 bp,개방열독광장도위534 bp,추측편마단백함유177개안기산,분자량위19.95 kD,등전점위6.9。통과여기타식물 PEBP 단백서렬적비대,발현화생 FT 기인속우 FT-Like 가족,가능재공제식물개화중기중요작용。
FT (Flowering Locus T ) encoding florigens plays important roles in the control of the transi-tion between vegetative and reproductive growth and promoting flowering.In this study, a cDNA encoding the FT homologue was isolated from peanut (Arachis hypogaea ) by RT-PCR and named as AhFT . Sequence analysis indicated that the cDNA of AhFT was 893 bp long and contained a single open reading frame with 534 bp in length.The predicted AhFT protein has 177 amino acids with an estimated molecular mass of 19.95 kD and an isoelectric point of 6.9.Phylogenetic analysis showed AhFT belonged to the FT-like subfamily.These results indicate AhFT may play important roles in flowering.
