CAJ | 학술논문

钙调蛋白（ calmodulin，CaM）是一种钙离子（ Ca2＋）结合蛋白，在钙信号传导过程中起重要作用。文章扩增到花刺参（Stichopus monotuberculatus）钙调蛋白（命名为StmCaM）cDNA全长序列。StmCaM cDNA全长1394 bp，其中5＇UTR长138 bp，3＇UTR长806 bp，ORF为450 bp。ORF推导出的StmCaM含149个氨基酸（包含起始蛋白酸），其预测分子量约为16.7 kDa，理论等电点为4.1。StmCaM氨基酸序列与其他物种的钙调蛋白高度相似，相似度百分比为95.9%~98.0%。采用realtime PCR分析StmCaM基因在花刺参不同组织中的表达，结果显示其在呼吸树中表达量最高，体腔细胞、肠次之，而体壁中表达量最低，几乎检测不到。
개조단백（ calmodulin，CaM）시일충개리자（ Ca2＋）결합단백，재개신호전도과정중기중요작용。문장확증도화자삼（Stichopus monotuberculatus）개조단백（명명위StmCaM）cDNA전장서렬。StmCaM cDNA전장1394 bp，기중5＇UTR장138 bp，3＇UTR장806 bp，ORF위450 bp。ORF추도출적StmCaM함149개안기산（포함기시단백산），기예측분자량약위16.7 kDa，이론등전점위4.1。StmCaM안기산서렬여기타물충적개조단백고도상사，상사도백분비위95.9%~98.0%。채용realtime PCR분석StmCaM기인재화자삼불동조직중적표체，결과현시기재호흡수중표체량최고，체강세포、장차지，이체벽중표체량최저，궤호검측불도。
Calmodulin,a calcium ion binding protein, plays an important role in calcium signal transduction system. This study cloned the cDNA of calmodulin from Stichopus monotuberculatus( named as StmCaM)by using homology cloning method and RACE approach. StmCaM cDNA comprised 1 394 nucleotides,which contained a 138-bp 5'UTR,an 806-bp 3'UTR and a 450-bp ORF. StmCaM deduced from this ORF contained 149 amino acids including the initial Met,whose estimated molecular mass was 16. 7 kDa and theoretical isoelectric point was 4. 1. The amino acid sequences of StmCaM showed high similarity with other species'CaMs,ran-ging from 95. 9% to 98. 0%. By utilizing real-time RCR to detect the expression pattern of CaM from different tissues of S. monotuberculatus,we found that respiratory tree had the highest mRNA expression level,followed by coelomocyte and intestine. However,body wall had the lowest expression level,which could hardly be detected.