CAJ | 학술논문

研究了基于两级人工湿地的温室循环水系统中，养殖密度对鲫（ Carassius cuvieri）生长、脏器系数、血清生理免疫指标及对嗜水气单胞菌（ Aeromonas hydrophila）抵抗力的影响。试验设2 kg·m-3、4 kg·m-3、8 kg·m-3和16 kg·m-3共4种密度组，每组2个平行，养殖68 d。结果显示：1）各养殖密度下鲫生长及脏器系数不存在显著差异；2）与2 kg·m-3组鲫相比，16 kg·m-3组鲫血清丙二醛（ MDA）质量摩尔浓度显著升高，溶菌酶（ LSZ）、酸性磷酸酶（ ACP）和碱性磷酸酶（ AKP）活性显著降低，超氧化物歧化酶（ SOD）活性和总蛋白质量浓度虽有不同程度升高，但差异不显著；3）高密度组鲫对嗜水气单胞菌的抵抗力较低密度组弱。以上结果表明，如果以生长为考量指标，温室湿地循环水系统中鲫养殖密度可达16 kg·m-3，但如此高的密度会对鲫免疫力造成负面影响。
연구료기우량급인공습지적온실순배수계통중，양식밀도대즉（ Carassius cuvieri）생장、장기계수、혈청생리면역지표급대기수기단포균（ Aeromonas hydrophila）저항력적영향。시험설2 kg·m-3、4 kg·m-3、8 kg·m-3화16 kg·m-3공4충밀도조，매조2개평행，양식68 d。결과현시：1）각양식밀도하즉생장급장기계수불존재현저차이；2）여2 kg·m-3조즉상비，16 kg·m-3조즉혈청병이철（ MDA）질량마이농도현저승고，용균매（ LSZ）、산성린산매（ ACP）화감성린산매（ AKP）활성현저강저，초양화물기화매（ SOD）활성화총단백질량농도수유불동정도승고，단차이불현저；3）고밀도조즉대기수기단포균적저항력교저밀도조약。이상결과표명，여과이생장위고량지표，온실습지순배수계통중즉양식밀도가체16 kg·m-3，단여차고적밀도회대즉면역력조성부면영향。
To investigate the effects of stocking density on the growth,organ coefficient,physiology-immune parameters in serum and the resistance against Aeromonas hydrophila of Carassius cuvieri reared in an indoor recirculating aquaculture system with 2-stage con-structed wetlands as filters,we cultured C. cuvieri for 68 d at various stocking densities of 2 kg·m-3 ,4 kg·m-3 ,8 kg·m-3 and 16 kg ·m-3( each density had two parallel groups). The results show that:1 )no significant difference was found among the four density-groups on growth and organ coefficients of C. cuvieri ;2)compared with 2 kg·m-3 density group,the concentration of malondialde-hyde( MDA)in serum of 16 kg·m-3 density group significantly increased,while the activities of serum lysozyme( LSZ),acid phos-phatase( ACP)and alkaline phosphatase( AKP)significantly decreased. The activity of superoxide dismutase( SOD)and concentra-tion of total protein were not significantly different although there was a certain degree of elevation;3 )the resistance of C. cuvieri a-gainst A. hydrophila was weaker in higher-density groups than that in lower-density groups. It is revealed that if considering growth as an indicator,stocking density of C. cuvieri could increase to 16 kg·m-3 when reared in indoor recirculating aquaculture system with 2-stage constructed wetlands as filters. However,it should be noted that there would be adverse effect of the immunity of C. cuvieri at such a high stocking density.