福建林学院学报
福建林學院學報
복건림학원학보
JOURNAL OF FUJIAN COLLEGE OF FORESTRY
2014年
4期
333-338
,共6页
金龟子绿僵菌%假眼小绿叶蝉%致病力%菌株筛选
金龜子綠僵菌%假眼小綠葉蟬%緻病力%菌株篩選
금구자록강균%가안소록협선%치병력%균주사선
Metarhizium anisopliae (Metschnikoff)%Empoasca vitis Gothe%pathogenicity%strain screening
采用喷雾法测定8株金龟子绿僵菌菌株对假眼小绿叶蝉的致病力。结果表明：以浓度（1±0．5）×107孢子· mL-1的绿僵菌孢子悬液接种12 d后，各菌株对假眼小绿叶蝉均有一定的致病力，校正死亡率和僵虫率分别为（43．08±0．76）％-（76．92±1．72）％和（40．67±1．25）％-（76．67±1．55）％，LT50为6．76-11．48 d。其中，Ma1775菌株对假眼小绿叶蝉致病力最强，校正死亡率和僵虫率分别为（76．92±1．72）％和（76．67±1．55）％，LT50为6．76 d，6 d的LC50为4．1563×107孢子· mL-1。通过时间-剂量-死亡率模型参数估算，Ma1775菌株对该虫致死效应较强的时间段为3-5 d，该菌株在假眼小绿叶蝉的生物防治中有很大的应用潜力。
採用噴霧法測定8株金龜子綠僵菌菌株對假眼小綠葉蟬的緻病力。結果錶明：以濃度（1±0．5）×107孢子· mL-1的綠僵菌孢子懸液接種12 d後，各菌株對假眼小綠葉蟬均有一定的緻病力，校正死亡率和僵蟲率分彆為（43．08±0．76）％-（76．92±1．72）％和（40．67±1．25）％-（76．67±1．55）％，LT50為6．76-11．48 d。其中，Ma1775菌株對假眼小綠葉蟬緻病力最彊，校正死亡率和僵蟲率分彆為（76．92±1．72）％和（76．67±1．55）％，LT50為6．76 d，6 d的LC50為4．1563×107孢子· mL-1。通過時間-劑量-死亡率模型參數估算，Ma1775菌株對該蟲緻死效應較彊的時間段為3-5 d，該菌株在假眼小綠葉蟬的生物防治中有很大的應用潛力。
채용분무법측정8주금구자록강균균주대가안소록협선적치병력。결과표명：이농도（1±0．5）×107포자· mL-1적록강균포자현액접충12 d후，각균주대가안소록협선균유일정적치병력，교정사망솔화강충솔분별위（43．08±0．76）％-（76．92±1．72）％화（40．67±1．25）％-（76．67±1．55）％，LT50위6．76-11．48 d。기중，Ma1775균주대가안소록협선치병력최강，교정사망솔화강충솔분별위（76．92±1．72）％화（76．67±1．55）％，LT50위6．76 d，6 d적LC50위4．1563×107포자· mL-1。통과시간-제량-사망솔모형삼수고산，Ma1775균주대해충치사효응교강적시간단위3-5 d，해균주재가안소록협선적생물방치중유흔대적응용잠력。
The virulence of 8 strains of Metarhizium anisopliae against Empoasca vitis was determined by spraying.The results showed that various strains of M.anisopliae had certain virulence against E.vitis after inoculation with (1 ±0.5) ×107 spores· mL-1 for 12 days.The ranges of the corrected mortality and cadaver rate were from (43.08 ±0.76)% to (76.92 ±1.72)%, and from (40.67 ±1.25)% to (76.67 ±1.55)%, respectively.The median lethal time ( LT50 ) was from 6.76 days to 11.48 days.When various strains were compared, Ma1775 strain exhibited the most effective virulence against E.vitis, causing a corrected mortality of (76.92 ±1.72)%, the cadaver rate of (76.67 ±1.55)% and the median lethal time ( LT50 ) of 6.76 days.The LC50 of Ma1775 strain to E.vitis on the 6th day was 4.156 3 ×107 spores· mL-1 .A time-dose-mortality model was employed to estimate parameters of time and dose, which showed that Ma1775 strain had a high lethal effect on E.vitis during between 3 and 5 days in the treatment, so Ma1775 strain has been proved to be a great potential application in biological control to E.vitis.
