CAJ | 학술논문

为给抗草铵膦转基因水稻‘Y0003’商业化生产后除草剂的安全高效施用提供科学依据，探索了该转基因直播稻田草铵膦的最佳施用时期和施用剂量。研究了在直播稻田3～4叶期、5～6叶期以及分蘖期施用0．225、0．45和0．9 g/hm2草铵膦（有效成分，下同）后，草铵膦对杂草的控制效果和对转基因水稻生长的影响。在测试的3个施药时期，施用0．225 g/hm2草铵膦对杂草防除效果都很差，水稻减产严重；在水稻3～4叶期和分蘖期施用0．45和0．9 g/hm2草铵膦对杂草的防除效果也较差，水稻生长受到严重影响，与无草对照相比水稻产量显著下降。但在水稻5～6叶期施用0．45和0．9 g/hm2草铵膦对主要杂草的防效均在90％以上，且对水稻安全。因此建议抗草铵膦转基因水稻‘Y0003’直播田在水稻5～6叶期施用0．45～0．9 g/hm2草铵膦来控制田间杂草。
위급항초안련전기인수도‘Y0003’상업화생산후제초제적안전고효시용제공과학의거，탐색료해전기인직파도전초안련적최가시용시기화시용제량。연구료재직파도전3～4협기、5～6협기이급분얼기시용0．225、0．45화0．9 g/hm2초안련（유효성분，하동）후，초안련대잡초적공제효과화대전기인수도생장적영향。재측시적3개시약시기，시용0．225 g/hm2초안련대잡초방제효과도흔차，수도감산엄중；재수도3～4협기화분얼기시용0．45화0．9 g/hm2초안련대잡초적방제효과야교차，수도생장수도엄중영향，여무초대조상비수도산량현저하강。단재수도5～6협기시용0．45화0．9 g/hm2초안련대주요잡초적방효균재90％이상，차대수도안전。인차건의항초안련전기인수도‘Y0003’직파전재수도5～6협기시용0．45～0．9 g/hm2초안련래공제전간잡초。
In order to provide scientific evidence for safety and effective application of glufosinate-ammonium,the optimum spraying period and effective dosage of glufosinate-ammonium was explored in direct-seeded rice field with glufosinate-ammonium-resistant (GR)transgenic rice ‘Y0003’.Different dosages of glufosinate-ammonium were applied at different spraying periods (three to four leaf stage,five to six leaf stage and tillering stage)in the direct-seeded rice field,and the effect of different spraying periods and dosage of glufosinate-ammonium on weed control and growth of GR transgenic rice were determined.In direct-seeded field,the weed control efficiency were low,and rice yield was reduced significantly at dosage of 0.225 g/hm2 (active ingredient,the same as fol-lows)under three spraying periods.Weed control efficiencies were also low at the dosage of 0.45 g /hm2 and 0.9 g/hm2 under three to four leaf stage and tillering stage.The growth of transgenic rice was inhibited and rice yield reduced significantly compared with the weed-free control.Whereas weed control efficiencies of 0.45 g/hm2 and 0.9 g/hm2 at five to six leaf stage was more than 90%,without injury on rice and influence on the rice yield. Therefore application of 0.45-0.9 g/hm2 glufosinate-ammonium at five to six leaf stage was recommended for weed control in direct-seeded GR transgenic rice ‘Y0003’field.