中国血吸虫病防治杂志
中國血吸蟲病防治雜誌
중국혈흡충병방치잡지
CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL
2014年
5期
510-513
,共4页
曲国立%戴建荣%邢云天%汪伟%杨振坤%赵正阳%郭娜%孙乐平%梁幼生
麯國立%戴建榮%邢雲天%汪偉%楊振坤%趙正暘%郭娜%孫樂平%樑幼生
곡국립%대건영%형운천%왕위%양진곤%조정양%곽나%손악평%량유생
日本血吸虫%预警%监测%扩展剂%C-6膜%尾蚴
日本血吸蟲%預警%鑑測%擴展劑%C-6膜%尾蚴
일본혈흡충%예경%감측%확전제%C-6막%미유
Schistosoma japonicum%Forecast%Surveillance%Expanding agents%C-6 film%Cercaria
目的：探索水面血吸虫尾蚴富集方法，并与已有疫水检测技术相结合，建立新型快速敏感的疫水检测方法。方法选取大豆油、汽油、试剂煤油、异佛尔酮等进行扩展剂筛选。借助扩展剂在水面扩散时所形成的推力，对水面漂浮的尾蚴进行富集，然后采用PE吸附膜及C-6膜粘附富集的尾蚴，快速确定水体的感染性。同时探索扩展剂剂量与扩散半径之间的关系。结果汽油、试剂煤油、异佛尔酮均可作为扩展剂进行实验，异佛尔酮的扩散效果最优。采用扩展剂富集后进行吸附膜粘取，可显著提高水中尾蚴检出率。结论新方法可以有效提高感染性疫水的检出率，适合低感染度水体检测。
目的：探索水麵血吸蟲尾蚴富集方法，併與已有疫水檢測技術相結閤，建立新型快速敏感的疫水檢測方法。方法選取大豆油、汽油、試劑煤油、異彿爾酮等進行擴展劑篩選。藉助擴展劑在水麵擴散時所形成的推力，對水麵漂浮的尾蚴進行富集，然後採用PE吸附膜及C-6膜粘附富集的尾蚴，快速確定水體的感染性。同時探索擴展劑劑量與擴散半徑之間的關繫。結果汽油、試劑煤油、異彿爾酮均可作為擴展劑進行實驗，異彿爾酮的擴散效果最優。採用擴展劑富集後進行吸附膜粘取，可顯著提高水中尾蚴檢齣率。結論新方法可以有效提高感染性疫水的檢齣率，適閤低感染度水體檢測。
목적：탐색수면혈흡충미유부집방법，병여이유역수검측기술상결합，건립신형쾌속민감적역수검측방법。방법선취대두유、기유、시제매유、이불이동등진행확전제사선。차조확전제재수면확산시소형성적추력，대수면표부적미유진행부집，연후채용PE흡부막급C-6막점부부집적미유，쾌속학정수체적감염성。동시탐색확전제제량여확산반경지간적관계。결과기유、시제매유、이불이동균가작위확전제진행실험，이불이동적확산효과최우。채용확전제부집후진행흡부막점취，가현저제고수중미유검출솔。결론신방법가이유효제고감염성역수적검출솔，괄합저감염도수체검측。
Objective To explore the enrichment technique of Schistosoma japonicum cercariae on the water surface,so as to establish a new method combined with the existing technology to detect the cercarial infested water body quickly and sensitive-ly. Methods Soybean oil,gasoline,kerosene and isophorone were screened as expanding agents. The cercariae were enriched by the thrust of the expanding agents when diffusing on the water surface,and PE adsorption film and C-6 film were applied to seize them so as to determine the infectivity of the water quickly. The relationship between the dose of expanding agents and dif-fusion radius were explored. Results Gasoline,kerosene and isophorone were suitable expanding agents,and the diffusion ef-fect of isophorone was the best. After the enrichment by the expanding agents,the detection rate of cercariae of the method seiz-ing cercariae with the film significantly improved in the water. Conclusion This new method could effectively improve the de-tection rate of the cercarial infested water and is suitable for the low-degree infested water.
