世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
9期
1974-1979
,共6页
郑晓珂%张娜%王晓帆%赫金丽%曹彦刚%李玲玲%袁培培%刘小盼%娄永%李鹏飞%冯卫生
鄭曉珂%張娜%王曉帆%赫金麗%曹彥剛%李玲玲%袁培培%劉小盼%婁永%李鵬飛%馮衛生
정효가%장나%왕효범%혁금려%조언강%리령령%원배배%류소반%루영%리붕비%풍위생
薏苡仁%雌激素样活性%植物雌激素%子宫系数MCF-7%雌激素受体
薏苡仁%雌激素樣活性%植物雌激素%子宮繫數MCF-7%雌激素受體
의이인%자격소양활성%식물자격소%자궁계수MCF-7%자격소수체
Coicis Semen%estrogenic activity%phytoestrogen%uterus index%MCF-7 cell%estrogen receptor
目的：对薏苡仁水煎液进行雌激素样活性筛选，探讨其作用机制。方法：首先通过小鼠子宫增重实验和人乳腺癌细胞（MCF-7）增殖实验对薏苡仁水煎液进行雌激素样活性筛选；以质粒pERE-TAL-luc、pβgal-Control、pCXN2-hERα或pCXN2-hERβ共转染的HEK293细胞株为模型，采用以ERE调控的报告基因luc瞬时表达检测技术，评价薏苡仁水煎液雌激素样作用的信号通路。结果：薏苡仁水煎液可显著的诱导性未成熟小鼠子宫系数的增加（P<0.01），且在去雌激素的培养基中能够显著促进MCF-7细胞的增殖（P<0.01）； ERE调控的报告基因瞬时表达结果显示，分别由ERα和ERβ介导时，与正常组相比，薏苡仁水煎液的标准化荧光素酶活性明显升高（P<0.05或P<0.01）。结论：薏苡仁水煎液可诱导性未成熟小鼠子宫系数的增加，在去雌激素的培养基中诱导MCF-7人乳腺癌细胞的增殖，首次发现薏苡仁具有雌激素样作用，且主要是通过ERβ介导发挥雌激素样作用。
目的：對薏苡仁水煎液進行雌激素樣活性篩選，探討其作用機製。方法：首先通過小鼠子宮增重實驗和人乳腺癌細胞（MCF-7）增殖實驗對薏苡仁水煎液進行雌激素樣活性篩選；以質粒pERE-TAL-luc、pβgal-Control、pCXN2-hERα或pCXN2-hERβ共轉染的HEK293細胞株為模型，採用以ERE調控的報告基因luc瞬時錶達檢測技術，評價薏苡仁水煎液雌激素樣作用的信號通路。結果：薏苡仁水煎液可顯著的誘導性未成熟小鼠子宮繫數的增加（P<0.01），且在去雌激素的培養基中能夠顯著促進MCF-7細胞的增殖（P<0.01）； ERE調控的報告基因瞬時錶達結果顯示，分彆由ERα和ERβ介導時，與正常組相比，薏苡仁水煎液的標準化熒光素酶活性明顯升高（P<0.05或P<0.01）。結論：薏苡仁水煎液可誘導性未成熟小鼠子宮繫數的增加，在去雌激素的培養基中誘導MCF-7人乳腺癌細胞的增殖，首次髮現薏苡仁具有雌激素樣作用，且主要是通過ERβ介導髮揮雌激素樣作用。
목적：대의이인수전액진행자격소양활성사선，탐토기작용궤제。방법：수선통과소서자궁증중실험화인유선암세포（MCF-7）증식실험대의이인수전액진행자격소양활성사선；이질립pERE-TAL-luc、pβgal-Control、pCXN2-hERα혹pCXN2-hERβ공전염적HEK293세포주위모형，채용이ERE조공적보고기인luc순시표체검측기술，평개의이인수전액자격소양작용적신호통로。결과：의이인수전액가현저적유도성미성숙소서자궁계수적증가（P<0.01），차재거자격소적배양기중능구현저촉진MCF-7세포적증식（P<0.01）； ERE조공적보고기인순시표체결과현시，분별유ERα화ERβ개도시，여정상조상비，의이인수전액적표준화형광소매활성명현승고（P<0.05혹P<0.01）。결론：의이인수전액가유도성미성숙소서자궁계수적증가，재거자격소적배양기중유도MCF-7인유선암세포적증식，수차발현의이인구유자격소양작용，차주요시통과ERβ개도발휘자격소양작용。
This study was aimed to investigate the estrogenic effects ofCoicis Semen in order to preliminarily discuss the mechanism. Mouse uterine weight test and MCF-7 cell proliferation assay were used to evaluate the estrogenic effects ofC. Semen. Reporter gene assays were adopted to explore the action mechanism ofC. Semen. In reporter gene assay, HEK293 cells were co-transfected with pERE-TAL-luc, pβgal-Control, pCXN2-hERα, or pCXN2-hERβ. And the expression of reporter gene luc was controlled by ERE. Mouse uterine weight test showed that compared with the control group, the aqueous extracts ofC. Semen can increase the uterus index of premature female mice (P<0.01). It can significantly promote the proliferation of MCF-7 cells in the medium without estrogen (P<0.01). The reporter gene controlled by ERE technology showed thatwhen mediated by ERα or ERβ respectively, the normalized luciferase activity of aqueous extracts ofC. Semen was significantly higher than activity of the control group (P< 0.05 orP< 0.01). It was concluded that the aqueous extracts ofC.Semen can increase the uterus index of premature female mice and promote the proliferation of MCF-7 cells in the medium without estrogen. We found the estrogenic effects ofC. Semen for the first time. And the estrogenic effects ofC. Semen were mainly mediated by ERβ.
