世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
9期
1951-1956
,共6页
王小兰%赫金丽%张国顺%孙亚萍%曹彦刚%张娜%李玲玲%袁培培%王绅%匡海学%郑晓珂
王小蘭%赫金麗%張國順%孫亞萍%曹彥剛%張娜%李玲玲%袁培培%王紳%劻海學%鄭曉珂
왕소란%혁금려%장국순%손아평%조언강%장나%리령령%원배배%왕신%광해학%정효가
桑白皮%化学拆分组分%止咳%祛痰%平喘%药理研究
桑白皮%化學拆分組分%止咳%祛痰%平喘%藥理研究
상백피%화학탁분조분%지해%거담%평천%약리연구
Mori Cortex%chemical split fractions%antitussive%expectorant%antiasthma%pharmacological research
目的：研究桑白皮水煎液及化学拆分组分的止咳祛痰平喘作用。方法：采用氨水诱咳法观察止咳作用；小鼠酚红排泌法观察祛痰作用；组胺与乙酰胆碱引喘法观察平喘作用；离体气管法观察解痉挛作用。结果：与空白组比较，桑白皮水煎液低、中、高剂量组均能显著减少小鼠咳嗽次数、增加小鼠呼吸道的酚红排泌量、延长豚鼠哮喘潜伏期、提高解痉率，以中剂量效果最好。各化学拆分组分与空白组相比，桑白皮30%、50%乙醇组分能明显减少浓氨水引起小鼠咳嗽的次数并延长咳嗽潜伏期、增加小鼠呼吸道的酚红排泌量（P<0.05或P<0.01），30%乙醇组分、脂肪油组分能延长豚鼠哮喘潜伏期（P<0.05或P<0.01），此外，30%乙醇组分能使气管收缩幅度明显降低，具有一定的解痉挛作用。结论：桑白皮水煎液具有较好的止咳、祛痰、平喘作用，其有效部位为30%乙醇组分，用量相当于中剂量的1/3，其止咳、祛痰、平喘作用最为显著，与水煎液中剂量作用相当。
目的：研究桑白皮水煎液及化學拆分組分的止咳祛痰平喘作用。方法：採用氨水誘咳法觀察止咳作用；小鼠酚紅排泌法觀察祛痰作用；組胺與乙酰膽堿引喘法觀察平喘作用；離體氣管法觀察解痙攣作用。結果：與空白組比較，桑白皮水煎液低、中、高劑量組均能顯著減少小鼠咳嗽次數、增加小鼠呼吸道的酚紅排泌量、延長豚鼠哮喘潛伏期、提高解痙率，以中劑量效果最好。各化學拆分組分與空白組相比，桑白皮30%、50%乙醇組分能明顯減少濃氨水引起小鼠咳嗽的次數併延長咳嗽潛伏期、增加小鼠呼吸道的酚紅排泌量（P<0.05或P<0.01），30%乙醇組分、脂肪油組分能延長豚鼠哮喘潛伏期（P<0.05或P<0.01），此外，30%乙醇組分能使氣管收縮幅度明顯降低，具有一定的解痙攣作用。結論：桑白皮水煎液具有較好的止咳、祛痰、平喘作用，其有效部位為30%乙醇組分，用量相噹于中劑量的1/3，其止咳、祛痰、平喘作用最為顯著，與水煎液中劑量作用相噹。
목적：연구상백피수전액급화학탁분조분적지해거담평천작용。방법：채용안수유해법관찰지해작용；소서분홍배비법관찰거담작용；조알여을선담감인천법관찰평천작용；리체기관법관찰해경련작용。결과：여공백조비교，상백피수전액저、중、고제량조균능현저감소소서해수차수、증가소서호흡도적분홍배비량、연장돈서효천잠복기、제고해경솔，이중제량효과최호。각화학탁분조분여공백조상비，상백피30%、50%을순조분능명현감소농안수인기소서해수적차수병연장해수잠복기、증가소서호흡도적분홍배비량（P<0.05혹P<0.01），30%을순조분、지방유조분능연장돈서효천잠복기（P<0.05혹P<0.01），차외，30%을순조분능사기관수축폭도명현강저，구유일정적해경련작용。결론：상백피수전액구유교호적지해、거담、평천작용，기유효부위위30%을순조분，용량상당우중제량적1/3，기지해、거담、평천작용최위현저，여수전액중제량작용상당。
This study was aimed to investigate the antitussive, expectorant and antiasthmatic effects of aqueous extracts and the chemical split fractions ofMori Cortex. Cough mice models induced by ammonia water were used to observe the effect on arresting cough. The phenol red expectoration method in mice was used to observe the effect on expelling phlegm. Histamine and acetylcholine mixture induced asthma model was used to observe the effect on relieving asthma. Isolated trachea model was used to observe the effect on relieving spasm. Compared with the control group, the low, medium and high doses of aqueous extracts ofM. Cortex can obviously decrease the frequency of cough, increase phenol red output of trachea in mice, prolong the latent period of asthma in guinea pigs, and increase the antispasmodic rate. The medium dose had the best effect. The comparison between different chemical split fractions ofM. Cortex and the control group showed that the 30% and 50% ethanol fraction ofM. Cortex can obviously decrease the frequency of cough and prolong the latent period of cough induced by ammonia water in mice, increase phenol red output of trachea in mice (P<0.05 orP<0.01); and 30% ethanol fraction and fatty oil fraction can prolong the latent period of asthma in guinea pigs (P< 0.05 orP<0.01). In addition, 30% ethanol fraction can obviously reduce the degree of tracheal contraction. It had certain effect of relieving spasm. It was concluded that aqueous extracts ofM. Cortex had better effects on relieving cough, expectorant and antiasthma. The effective part was the 30% ethanol fraction, which was the dose equivalent of 1/3 of the medium dose. It had significant effect on relieving cough, expectorant and antiasthma. The effect was equivalent to the medium dose of aqueous extracts of M. Cortex.
