CAJ | 학술논문

目的：探讨桑白皮的利尿作用及发挥利尿作用的有效部位。方法：采用大鼠代谢笼法，首先观察桑白皮水煎液的利尿作用，连续给药5天，筛选出发挥利尿作用的最佳剂量，并用苦味酸法检测给药5天后血清和尿液中肌酐的含量；进而研究桑白皮各化学拆分组分的利尿作用，确定桑白皮发挥利尿作用的有效部位。结果：桑白皮水煎液给药第3-5天后，与对照组相比，大鼠尿量明显增加（P<0.05或P<0.01），且中剂量利尿效果最为显著（P<0.01）；桑白皮水煎液对血肌酐、尿肌酐含量没有显著影响；各化学拆分组分利尿结果显示，与对照组相比，30%乙醇组分和脂肪油组分利尿作用最好（P<0.01）。结论：桑白皮水煎液具有明显的利尿作用，且发挥利尿作用的物质基础主要集中在30%乙醇组分和脂肪油组分。
목적：탐토상백피적이뇨작용급발휘이뇨작용적유효부위。방법：채용대서대사롱법，수선관찰상백피수전액적이뇨작용，련속급약5천，사선출발휘이뇨작용적최가제량，병용고미산법검측급약5천후혈청화뇨액중기항적함량；진이연구상백피각화학탁분조분적이뇨작용，학정상백피발휘이뇨작용적유효부위。결과：상백피수전액급약제3-5천후，여대조조상비，대서뇨량명현증가（P<0.05혹P<0.01），차중제량이뇨효과최위현저（P<0.01）；상백피수전액대혈기항、뇨기항함량몰유현저영향；각화학탁분조분이뇨결과현시，여대조조상비，30%을순조분화지방유조분이뇨작용최호（P<0.01）。결론：상백피수전액구유명현적이뇨작용，차발휘이뇨작용적물질기출주요집중재30%을순조분화지방유조분。
This study was aimed to investigate the diuretic effect ofMori Cortex and to identify effective fractions inM. Cortex. Metabolic cages were used to firstly observe the diuretic effect ofM. Cortex aqueous extracts on rats. Medications were continuously given for 5 days to screen for the best dosage of diuretic effect. Picric acid assays were used to detect creatinine levels in serum and urine after 5-day medication. Then, the diuretic effect of each chemical split fraction inM. Cortex was studied in order to indentify the effective parts. The results showed that after administration ofM. Cortex aqueous extracts for 3 to 5 days, compared with the control group, there was a significant diuretic effect on rats (P<0.05 orP<0.01). And the medium-dose ofM. Cortex aqueous extracts showed the best effect (P<0.01). However,M. Cortex aqueous extracts had no significant effect on creatinine levels in serum and urine. Assays of diuretic effect of chemical split fractions ofM.Cortex indicated that compared with the control group, 30% ethanol fraction and fatty oil fraction had the best diuretic effect (P<0.01). It was concluded thatM.Cortex aqueous extracts had a significant diuretic effect. And the chemical fractions contributed mostly to this effect were mainly existed in the 30% ethanol fraction and fatty oil fraction.