CAJ | 학술논문

背景与目的：胃癌术后复发转移严重影响患者生存情况，SOX基因是经典Wnt信号通路的调控因子，其在胃癌术后复发及转移过程中可能发挥重要作用。本研究旨在探讨胃癌术后复发、远处转移患者肿瘤组织中SOX-2、β-catenin表达情况，探讨两者在胃癌术后复发及转移的作用。方法：采用免疫组化方法检测71例经手术切除胃癌患者的肿瘤组织中SOX-2和β-catenin蛋白表达情况，并分析其与临床病理特征和无病生存时间(disease free survival，DFS)的关系。结果：SOX-2在胃癌中的表达与胃癌复发转移、淋巴结浸润及分化程度有关(P=0.011，P=0.036，P=0.034)，与患者性别、年龄及T分期无关。β-catenin在胃癌中的表达与胃癌复发转移、淋巴结浸润及T分期有关(P=0.025，P=0.014，P=0.026)，与分化程度、患者性别及年龄无关。且二者均呈阳性表达者其术后复发转移率高于任意单阳性者，复发转移率分别为84%(21/25)和66.7%(24/36)，二者均阴性表达者其复发转移率为30%(3/10)。生存分析显示SOX-2和β-catenin的表达与患者DFS相关。结论：SOX-2、β-catenin表达可能是胃癌术后复发转移有效的预测因子，两者联合检测有利于预测胃癌患者术后复发转移。
배경여목적：위암술후복발전이엄중영향환자생존정황，SOX기인시경전Wnt신호통로적조공인자，기재위암술후복발급전이과정중가능발휘중요작용。본연구지재탐토위암술후복발、원처전이환자종류조직중SOX-2、β-catenin표체정황，탐토량자재위암술후복발급전이적작용。방법：채용면역조화방법검측71례경수술절제위암환자적종류조직중SOX-2화β-catenin단백표체정황，병분석기여림상병리특정화무병생존시간(disease free survival，DFS)적관계。결과：SOX-2재위암중적표체여위암복발전이、림파결침윤급분화정도유관(P=0.011，P=0.036，P=0.034)，여환자성별、년령급T분기무관。β-catenin재위암중적표체여위암복발전이、림파결침윤급T분기유관(P=0.025，P=0.014，P=0.026)，여분화정도、환자성별급년령무관。차이자균정양성표체자기술후복발전이솔고우임의단양성자，복발전이솔분별위84%(21/25)화66.7%(24/36)，이자균음성표체자기복발전이솔위30%(3/10)。생존분석현시SOX-2화β-catenin적표체여환자DFS상관。결론：SOX-2、β-catenin표체가능시위암술후복발전이유효적예측인자，량자연합검측유리우예측위암환자술후복발전이。
Background and purpose:The recurrence and metastasis of gastric cancer seriously affect survival in patients.SOX gene as a regulatory factor of the classical Wnt pathway, may play an important role in the process. This study was to explore the expression of stem cell marker SOX-2 and β-catenin in gastric cancer and to analyze the relationship with recurrence and metastasis after operation.Methods:Immunohistochemistry was used to detect the expression of SOX-2 and β-catenin in 71 tumor samples from 71 cases after surgery for gastric cancer. The correlation between SOX-2 and β-catenin expression and the clinicopathological characteristics of gastric cancer and disease-free survival was analyzed.Results:The SOX-2 protein expression was associated with metastasis, lymph node inifltration or differentiation (P=0.011,P=0.036,P=0.034) in the 71 gastric cancer, but not with gender, age or T stage. β-catenin expression was correlated with metastasis, lymph node invasion or T stage (P=0.025,P=0.014,P=0.026), but was not related to differentiation, gender or age. The survival analysis showed that SOX-2 and β-catenin expression was closely associated with prognosis of patients, and metastatic rate in positive expression was higher than that in negative expression.Conclusion:The expression of SOX-2 and β-catenin is associated with the development, recurrence, metastasis of gastric cancer and may be used as a useful prognostic parameter to predict overall survival.