中国癌症杂志
中國癌癥雜誌
중국암증잡지
CHINA ONCOLOGY
2014年
9期
669-675
,共7页
白夏楠%姜永冬%刘通%吴昊%张金锋%庞达
白夏楠%薑永鼕%劉通%吳昊%張金鋒%龐達
백하남%강영동%류통%오호%장금봉%방체
乳腺肿瘤%单核苷酸多态%遗传易感性%2q35 rs13387042%8q24 rs13281615
乳腺腫瘤%單覈苷痠多態%遺傳易感性%2q35 rs13387042%8q24 rs13281615
유선종류%단핵감산다태%유전역감성%2q35 rs13387042%8q24 rs13281615
Breast neoplasms%Single nucleotide polymorphism%Genetic susceptibility%2q35 rs13387042%8q24 rs13281615
背景与目的：乳腺癌作为中国女性最常见的恶性肿瘤，每年的新发数量和死亡数量分别占全世界的12.2%和9.6%，但与中国乳腺癌患者明显相关的基因多态位点至今尚不清楚。本研究旨在探讨2q35 rs13387042和8q24 rs13281615单核苷酸多态性与中国北方汉族绝经前妇女乳腺癌风险关系，为预防和治疗乳腺癌提供循证依据。方法：采用多重单碱基延伸单核苷酸多态性分型技术(SNaPshot)分析方法，检测了280例绝经前乳腺癌患者和287例绝经前正常对照者2q35 rs13387042和8q24 rs13281615多态性位点基因型，并比较不同基因型和等位基因与乳腺癌风险的关系。结果：2q35 rs13387042多态性位点基因型频率在乳腺癌和对照样本之间差异有统计学意义(P=0.017)；8q24 rs13281615多态性位点基因型频率在乳腺癌和对照样本之间差异无统计学意义(P=0.967)。Logistic回归分析结果显示，对于2q35 rs13387042位点，与GG相比，GA和GA+AA基因型携带者显著增加乳腺癌的患病风险(OR=1.793，95%CI：1.177～2.733，P=0.007；OR=1.691，95%CI：1.122～2.550， P=0.012)，而AA携带者与乳腺癌的患病风险无关(OR=0.572，95%CI：0.104～3.153，P=0.521)；与G等位基因相比，A等位基因显著增加乳腺癌的患病风险(OR=1.505，95%CI：1.033～2.193，P=0.033)。对于8q24 rs13281615位点，与AA相比，AG、GG和AG+GG基因型携带者与乳腺癌的患病风险无关(OR=0.992，95%CI：0.660～1.490，P=0.968；OR=1.047，95%CI：0.642～1.708，P=0.853；OR=1.007，95%CI：0.682～1.487， P=0.971)；与A等位基因相比，G等位基因不增加乳腺癌患病风险(OR=1.021，95%CI：0.809～1.288， P=0.863)。结论：本实验证实2q35 rs13387042多态性位点能够增加中国北方汉族绝经前妇女乳腺癌易感风险，而8q24 rs13281615多态性位点与中国北方汉族绝经前妇女乳腺癌易感性无明显相关性。
揹景與目的：乳腺癌作為中國女性最常見的噁性腫瘤，每年的新髮數量和死亡數量分彆佔全世界的12.2%和9.6%，但與中國乳腺癌患者明顯相關的基因多態位點至今尚不清楚。本研究旨在探討2q35 rs13387042和8q24 rs13281615單覈苷痠多態性與中國北方漢族絕經前婦女乳腺癌風險關繫，為預防和治療乳腺癌提供循證依據。方法：採用多重單堿基延伸單覈苷痠多態性分型技術(SNaPshot)分析方法，檢測瞭280例絕經前乳腺癌患者和287例絕經前正常對照者2q35 rs13387042和8q24 rs13281615多態性位點基因型，併比較不同基因型和等位基因與乳腺癌風險的關繫。結果：2q35 rs13387042多態性位點基因型頻率在乳腺癌和對照樣本之間差異有統計學意義(P=0.017)；8q24 rs13281615多態性位點基因型頻率在乳腺癌和對照樣本之間差異無統計學意義(P=0.967)。Logistic迴歸分析結果顯示，對于2q35 rs13387042位點，與GG相比，GA和GA+AA基因型攜帶者顯著增加乳腺癌的患病風險(OR=1.793，95%CI：1.177～2.733，P=0.007；OR=1.691，95%CI：1.122～2.550， P=0.012)，而AA攜帶者與乳腺癌的患病風險無關(OR=0.572，95%CI：0.104～3.153，P=0.521)；與G等位基因相比，A等位基因顯著增加乳腺癌的患病風險(OR=1.505，95%CI：1.033～2.193，P=0.033)。對于8q24 rs13281615位點，與AA相比，AG、GG和AG+GG基因型攜帶者與乳腺癌的患病風險無關(OR=0.992，95%CI：0.660～1.490，P=0.968；OR=1.047，95%CI：0.642～1.708，P=0.853；OR=1.007，95%CI：0.682～1.487， P=0.971)；與A等位基因相比，G等位基因不增加乳腺癌患病風險(OR=1.021，95%CI：0.809～1.288， P=0.863)。結論：本實驗證實2q35 rs13387042多態性位點能夠增加中國北方漢族絕經前婦女乳腺癌易感風險，而8q24 rs13281615多態性位點與中國北方漢族絕經前婦女乳腺癌易感性無明顯相關性。
배경여목적：유선암작위중국녀성최상견적악성종류，매년적신발수량화사망수량분별점전세계적12.2%화9.6%，단여중국유선암환자명현상관적기인다태위점지금상불청초。본연구지재탐토2q35 rs13387042화8q24 rs13281615단핵감산다태성여중국북방한족절경전부녀유선암풍험관계，위예방화치료유선암제공순증의거。방법：채용다중단감기연신단핵감산다태성분형기술(SNaPshot)분석방법，검측료280례절경전유선암환자화287례절경전정상대조자2q35 rs13387042화8q24 rs13281615다태성위점기인형，병비교불동기인형화등위기인여유선암풍험적관계。결과：2q35 rs13387042다태성위점기인형빈솔재유선암화대조양본지간차이유통계학의의(P=0.017)；8q24 rs13281615다태성위점기인형빈솔재유선암화대조양본지간차이무통계학의의(P=0.967)。Logistic회귀분석결과현시，대우2q35 rs13387042위점，여GG상비，GA화GA+AA기인형휴대자현저증가유선암적환병풍험(OR=1.793，95%CI：1.177～2.733，P=0.007；OR=1.691，95%CI：1.122～2.550， P=0.012)，이AA휴대자여유선암적환병풍험무관(OR=0.572，95%CI：0.104～3.153，P=0.521)；여G등위기인상비，A등위기인현저증가유선암적환병풍험(OR=1.505，95%CI：1.033～2.193，P=0.033)。대우8q24 rs13281615위점，여AA상비，AG、GG화AG+GG기인형휴대자여유선암적환병풍험무관(OR=0.992，95%CI：0.660～1.490，P=0.968；OR=1.047，95%CI：0.642～1.708，P=0.853；OR=1.007，95%CI：0.682～1.487， P=0.971)；여A등위기인상비，G등위기인불증가유선암환병풍험(OR=1.021，95%CI：0.809～1.288， P=0.863)。결론：본실험증실2q35 rs13387042다태성위점능구증가중국북방한족절경전부녀유선암역감풍험，이8q24 rs13281615다태성위점여중국북방한족절경전부녀유선암역감성무명현상관성。
Background and purpose:Breast cancer as one of the most common malignant tumor among women in China, it accounts for 12.2% of all newly diagnosed breast cancers and 9.6% of all deaths from breast cancer worldwide. The aim of this study was to investigate the relationship between single nucleotide polymorphisms(SNPs) in 2q35rs13387042and 8q24 rs13281615and the risk of breast cancer in Han premenopausal women of Northern China. Methods:280 patients with breast cancer and 287 healthy controls in premenopausal state were genotyped for SNP 2q35rs13387042and 8q24 rs13281615 by the SNaPshot method, and compared the different genotypes and alleles with relation to breast cancer risk.Results:Differences of 2q35 rs13387042 genotype frequencies between breast cancer and control were signiifcantly different (P=0.017). No statistically signiifcant difference of 8q24 rs13281615 genotype frequencies between breast cancers and controls was found (P=0.967). The results of logistic regression showed that the carriers of GA genotype and GA+ AA genotype increased risk for breast cancer compared to the carriers with 2q35 rs13387042 GG genotype(OR=1.793, 95%CI: 1.177-2.733,P=0.007;OR=1.691, 95%CI: 1.122-2.550,P=0.012), but not the carriers of AA genotype; Compared with G allele, A allele signiifcantly increased the risk of breast cancer(OR= 1.505, 95%CI: 1.033-2.193,P=0.033). The carriers of AG genotype or GG genotype or AG+GG genotype did not confer risk for breast cancer compared to the carriers with 8q24 rs13281615 AA genotype(OR=0.992, 95%CI: 0.660-1.490,P=0.968;OR=1.047, 95%CI: 0.642-1.708,P=0.853;OR=1.007, 95%CI: 0.682-1.487,P=0.971); Compared with A allele, G allele did not increase the risk of breast cancer(OR=1.021, 95%CI: 0.809-1.288,P=0.863).Conclusion:This experiment veriifed that 2q35 rs13387042 polymorphism site increased risk of breast cancer susceptibility among Han premenopausal women of Northern China. There was not any signiifcant association between 8q24 rs13281615 poly-morphism site and breast cancer susceptibility among Han premenopausal women of Northern China under the current sampling scale.
