CAJ | 학술논문

热激蛋白90(Heat shock protein 90，Hsp90)是植物应对不良环境胁迫产生的一类特定的抗逆蛋白。文章以番茄(Solanum lycopersicum L.)基因组数据为平台，借助生物信息学方法对Hsp90基因家族进行鉴定与分析。结果表明，番茄至少含有7个Hsp90基因，不均匀分布在6条染色体上，氨基酸序列长度为267~794aa，内含子数目为2~19；共线性分析发现两对基因(Hsp90-1和 Hsp90-3，Hsp90-5和 Hsp90-7)以片段重复形式存在。MEME(Multiple Em for Motif Elicitation)分析显示，番茄Hsp90基因编码的氨基酸序列具有多个保守基序；聚类分析揭示番茄、水稻(Oryza sativaL.)和拟南芥(Arabidopsis thaliana L.)Hsp90基因可以分为5组，存在3对直系同源基因和4对旁系同源基因；基于RNA-seq数据库表达分析发现，3个基因(Hsp90-5、Hsp90-6和Hsp90-7)在营养器官和生殖器官中表达量较高，4个基因(Hsp90-1、Hsp90-2、Hsp90-3和Hsp90-4)除在番茄转色后10 d的果实中表达量较高外，其余组织中表达量均较低；对Hsp90基因启动子序列进行分析，发现了多个参与植物对逆境胁迫的顺式作用元件，如 HSE、CCAAT-box。此外，qRT-PCR 检测结果表明，在叶片热胁迫条件下，番茄Hsp90基因的表达量均存在增强趋势，表明这些基因参与了番茄叶片应对高温胁迫的反应。研究结果为鉴定番茄Hsp90基因的功能和进化起源奠定了基础。
열격단백90(Heat shock protein 90，Hsp90)시식물응대불량배경협박산생적일류특정적항역단백。문장이번가(Solanum lycopersicum L.)기인조수거위평태，차조생물신식학방법대Hsp90기인가족진행감정여분석。결과표명，번가지소함유7개Hsp90기인，불균균분포재6조염색체상，안기산서렬장도위267~794aa，내함자수목위2~19；공선성분석발현량대기인(Hsp90-1화 Hsp90-3，Hsp90-5화 Hsp90-7)이편단중복형식존재。MEME(Multiple Em for Motif Elicitation)분석현시，번가Hsp90기인편마적안기산서렬구유다개보수기서；취류분석게시번가、수도(Oryza sativaL.)화의남개(Arabidopsis thaliana L.)Hsp90기인가이분위5조，존재3대직계동원기인화4대방계동원기인；기우RNA-seq수거고표체분석발현，3개기인(Hsp90-5、Hsp90-6화Hsp90-7)재영양기관화생식기관중표체량교고，4개기인(Hsp90-1、Hsp90-2、Hsp90-3화Hsp90-4)제재번가전색후10 d적과실중표체량교고외，기여조직중표체량균교저；대Hsp90기인계동자서렬진행분석，발현료다개삼여식물대역경협박적순식작용원건，여 HSE、CCAAT-box。차외，qRT-PCR 검측결과표명，재협편열협박조건하，번가Hsp90기인적표체량균존재증강추세，표명저사기인삼여료번가협편응대고온협박적반응。연구결과위감정번가Hsp90기인적공능화진화기원전정료기출。
Heat shock proteins 90 (Hsp90) are a kind of specific proteins in plant which were produced under environmental stresses. By referring to the tomato genome database, we identified and analyzedHsp90gene family members using bioinformatics methods. Results indicated that the tomato genome contained at least 7Hsp90 genes, which were distributed unevenly on 6 chromosomes. Amino acid sequence length of these proteins ranged from 267 to 794aa. Numbers of intron ranged from 2 to 19. Microsynteny analysis showed that twopairs ofHsp90genes (Hsp90-1andHsp90-3, Hsp90-5andHsp90-7) were identified by segment duplication. In addition, multiple conserva-tion motifs were found in Hsp90proteins. Phylogenetic analysis revealed thatHsp90 genes from tomato, rice and Arabidopsis can be divided into 5 groups. Three pair of orthologous genes and four pairs of homologous genes were found. Expression analysis based on RNA-seq showed that the expression of three genes (Hsp90-5,Hsp90-6 and Hsp90-7) was high in vegetable and reproductive organs, while the expression of other four genes (Hsp90-1,Hsp90-2, Hsp90-3 andHsp90-4) was relatively low except for its expression at the breaking stage of fruit. Analysis of promoter regions ofHsp90 genes showed that multiplecis-elements were involved in plant responses to biotic and abiotic stresses. The expression of 7 genes under heat stress was also detected by qRT-PCR. Expression of allHsp90 genes in tomato leaf was enhanced. The results indicated that these genes could be participated in tomato leaf response to heat stresses. Together, these results will lay a foundation for analyzingHsp90 gene function and molecular evolution in the future.